Rabbit Recombinant Monoclonal CD2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
IHC-P | |
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Human | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature. |
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CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5
Rabbit Recombinant Monoclonal CD2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
Purified from TCS by protein A/G.
ab272017 is the carrier-free version of Anti-CD2 antibody [SP304] ab227698.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.
The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.
CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.
CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human thymus tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human colon tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human stomach tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human uterus tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD2 using Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution in immunohistochemical analysis. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue sections labelling CD2 with Anti-CD2 antibody [SP304] ab227698 at 1/100 dilution. The section was incubated with Anti-CD2 antibody [SP304] ab227698 for 10 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on the human tonsil tissue, performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD2 antibody [SP304] ab227698).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com