AB275973

Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric)

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD20 antibody. Suitable for Flow Cyt and reacts with Human, Cynomolgus monkey, Rhesus monkey samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MS4A1 protein.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

3 Images

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Flow cytometry analysis of human lymphocytes labeling CD20 with ab275973 (panel B) compared with an isotype control (panel A) at a concentration of 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using an AlexaFluor^®488 conjugated Donkey anti-Rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Flow cytometry analysis of Rhesus monkey lymphocytes labeling CD20 with ab275973 (panel B) compared with an isotype control (panel A) at a concentration of 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using an AlexaFluor^®488 conjugated Donkey anti-Rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Flow cytometry analysis of cynomolgus monkey lymphocytes labeling CD20 with ab275973 (panel B) compared with an isotype control (panel A) at a concentration of 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using an AlexaFluor^®488 conjugated Donkey anti-Rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

10F381(rituximab)

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human, Cynomolgus monkey, Rhesus monkey

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11836

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "" } } }

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Product details

This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e17366 PubMed37426804

2023

circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Yuhao Lin,Chao Wang,Pengcheng Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric)

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Flow Cytometry - Anti-CD20 antibody [10F381(rituximab)] - Rabbit IgG (Chimeric) (AB275973)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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