AB244336

Anti-CD20 antibody

Name: Anti-CD20 antibody (ab244336) | Abcam
Brand: Abcam Limited
SKU: AB244336
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CD20 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MS4A1 aa 200-300.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human gastrointestinal tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Low expression as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody (AB244336)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody (AB244336)

PFA fixed, Triton X-100 permeabilized REH (Human cell line) cells labeling CD20 using ab244336 at 4 μg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human testis tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB244336)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD20 with ab244336 at a 1/1000 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-CD20 antibody (AB244336)

All lanes:

Western blot - Anti-CD20 antibody (ab244336) at 0.4 µg/mL

Lane 1:

Daudi cells

Lane 2:

U-2 OS cells

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MS4A1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P11836

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39088302

2024

Human gut microbiota-reactive DP8α Tregs prevent acute graft-versus-host disease in a CD73-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuelle Godefroy,Patrice Chevallier,Fabienne Haspot,Caroline Vignes,Véronique Daguin,Sylvia Lambot,Margaux Verdon,Margaux De Seilhac,Valentin Letailleur,Anne Jarry,Annabelle Pédron,Thierry Guillaume,Pierre Peterlin,Alice Garnier,Marie-Anne Vibet,Maxence Mougon,Amandine Le Bourgeois,Maxime Jullien,Francine Jotereau,Frédéric Altare

Cell death & disease 14:83 PubMed36739418

2023

VHL-HIF-2α axis-induced SEMA6A upregulation stabilized β-catenin to drive clear cell renal cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ji,Yuxin Xu,Mengru Xie,Xingbei He,Dexu Ren,Teng Qiu,Wenwen Liu,Zefeng Chen,Wen Shi,Zhen Zhang,Xiujun Wang,Weiling Wang,Jinming Ma,Qilan Qian,Aixin Jing,Xinhui Ma,Jingting Qin,Yuanyuan Ding,Ting Geng,Jiayan Yang,Zhichao Sun,Wei Liu,Shunfang Liu,Bin Liu

View all publications

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