AB27093

Anti-CD20 antibody

Name: Anti-CD20 antibody (ab27093) | Abcam
Brand: Abcam Limited
SKU: AB27093
Price: 440 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(10 Publications)

Anti-CD20 antibody (ab27093) is a rabbit polyclonal antibody detecting CD20 in Western Blot, IHC-P. Suitable for Human.

- Trusted since 2005

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB27093)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody (AB27093)

Formalin fixed, paraffin embedded human tonsil tissue slide stained with anti CD20 antibody (ab27093).

Unknown

Western blot - Anti-CD20 antibody (AB27093)

Lane 1:

Western blot - Anti-CD20 antibody (ab27093) at 1/25 dilution

Lane 1:

Western blot - Anti-CD20 antibody, prediluted (<a href='/en-us/products/primary-antibodies/cd20-antibody-prediluted-ab27092'>ab27092</a>) at 1/25 dilution

All lanes:

Raji cell lysate

Predicted band size: 33 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-CD20 antibody (ab27093) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of CD20?
Anti-CD20 (ab27093) specifically detects a band for CD20 (UniProt: P11836) at a molecular weight of 33kDa.

Trusted by the scientific community
Anti-CD20 (ab27093) was first used in a scientific publication in 2005 and has been cited over 10 times in peer-reviewed journals.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab27093 is an affinity purified IgG.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 13: PubMed38003099

2023

Histological Characteristics of Conjunctiva-Associated Lymphoid Tissue in Young and Adult Holstein Cattle.

Applications

Unspecified application

Species

Unspecified reactive species

Keigo Kosenda,Osamu Ichii,Yusuke Yamashita,Hiromichi Ohtsuka,Shigeo Fukuda,Yasuhiro Kon

Scientific reports 12:5120 PubMed35332215

2022

Evaluation of 41 single nucleotide polymorphisms in canine diffuse large B-cell lymphomas using MassARRAY.

Applications

Unspecified application

Species

Unspecified reactive species

Sirintra Sirivisoot,Tanit Kasantikul,Somporn Techangamsuwan,Araya Radtanakatikanon,Ken Chen,Tzu-Yin Lin,Anudep Rungsipipat

EBioMedicine 57:102850 PubMed32574962

2020

Immune-infiltration based signature as a novel prognostic biomarker in gastrointestinal stromal tumour.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe-Wei Wei,Jing Wu,Wei-Bin Huang,Jin Li,Xiao-Fang Lu,Yu-Jie Yuan,Wen-Jun Xiong,Xin-Hua Zhang,Wei Wang,Yu-Long He,Chang-Hua Zhang

Biomaterials 187:93-104 PubMed30312852

2018

Evaluation of the host immune response to decellularized lung scaffolds derived from α-Gal knockout pigs in a non-human primate model.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth C Stahl,Ryan W Bonvillain,Clint D Skillen,Brandon L Burger,Hidetaka Hara,Whayoung Lee,Cynthia B Trygg,Peter J Didier,Brooke F Grasperge,Nicholas C Pashos,Bruce A Bunnell,John Bianchi,David L Ayares,Kelly I Guthrie,Bryan N Brown,Thomas H Petersen

Blood advances 2:76-84 PubMed29365313

2018

The human IL-15 superagonist ALT-803 directs SIV-specific CD8 T cells into B-cell follicles.

Applications

IHC-P

Species

Rhesus monkey

Gabriela M Webb,Shengbin Li,Gwantwa Mwakalundwa,Joy M Folkvord,Justin M Greene,Jason S Reed,Jeffery J Stanton,Alfred W Legasse,Theodore Hobbs,Lauren D Martin,Byung S Park,James B Whitney,Emily K Jeng,Hing C Wong,Douglas F Nixon,R Brad Jones,Elizabeth Connick,Pamela J Skinner,Jonah B Sacha

PLoS pathogens 12:e1005924 PubMed27716848

2016

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

Applications

IHC (PFA fixed)

Species

Rhesus monkey

Brodie Miles,Shannon M Miller,Joy M Folkvord,David N Levy,Eva G Rakasz,Pamela J Skinner,Elizabeth Connick

Nature communications 6:8608 PubMed26482032

2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Brodie Miles,Shannon M Miller,Joy M Folkvord,Abigail Kimball,Mastooreh Chamanian,Amie L Meditz,Tessa Arends,Martin D McCarter,David N Levy,Eva G Rakasz,Pamela J Skinner,Elizabeth Connick

Journal of immunology (Baltimore, Md. : 1950) 193:5613-25 PubMed25362178

2014

Compartmentalization of simian immunodeficiency virus replication within secondary lymphoid tissues of rhesus macaques is linked to disease stage and inversely related to localization of virus-specific CTL.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Connick,Joy M Folkvord,Katherine T Lind,Eva G Rakasz,Brodie Miles,Nancy A Wilson,Mario L Santiago,Kimberly Schmitt,Edward B Stephens,Hyeon O Kim,Reece Wagstaff,Shengbin Li,Hadia M Abdelaal,Nathan Kemp,David I Watkins,Samantha MaWhinney,Pamela J Skinner

Leukemia 28:1163-7 PubMed24492323

2014

B-cell receptor pathway inhibitors affect CD20 levels and impair antitumor activity of anti-CD20 monoclonal antibodies.

Applications

Unspecified application

Species

Human

K Bojarczuk,M Siernicka,M Dwojak,M Bobrowicz,B Pyrzynska,P Gaj,M Karp,K Giannopoulos,D G Efremov,C Fauriat,J Golab,M Winiarska

Archives of neurology 66:44-53 PubMed19139298

2009

Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis.

Applications

IHC-Fr

Species

Human

Natalia M Moll,Michael B Cossoy,Elizabeth Fisher,Susan M Staugaitis,Barbara H Tucky,Anna M Rietsch,Ansi Chang,Robert J Fox,Bruce D Trapp,Richard M Ransohoff

View all publications

style

left-column

style

left-column

style

right-column