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AB171203

Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free

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(1 Publication)

Rat Monoclonal CD20 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

2 Images
Western blot - Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (AB171203)
  • WB

Supplier Data

Western blot - Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (AB171203)

All lanes:

Western blot - Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (ab171203)

Lane 1:

Balb/c thymus cell lysate

Lane 2:

A20 cell lysate

Predicted band size: 33 kDa

false

Flow Cytometry - Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (AB171203)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (AB171203)

Flow cytometric analysis of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor® 647 and 1.0 ug ab171203 followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

AISB12

Isotype

IgG2a

Carrier free

Yes

Reacts with

Mouse

Applications

WB, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>(in a final volume of 100 μL).</p><p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Assay dependent</p>" } } }
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Product details

Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:38810-38821 PubMed27246977

2016

Technetium-99m-labeled rituximab for use as a specific tracer of sentinel lymph node biopsy: a translational research study.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejuan Wang,Zhi Yang,Baohe Lin,Yan Zhang,Shizhen Zhai,Qichao Zhao,Qing Xie,Fei Liu,Xuedi Han,Jinfeng Li,Tao Ouyang
View all publications
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