AB194970

Anti-CD20 antibody - C-terminal

Name: Anti-CD20 antibody - C-terminal (ab194970) | Abcam
Brand: Abcam Limited
SKU: AB194970
Price: 630 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(6 Publications)

Goat Polyclonal CD20 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MS4A1 aa 250 to C-terminus.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody - C-terminal (AB194970)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody - C-terminal (AB194970)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD20 with ab194970 at 2.5 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody - C-terminal (AB194970)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling CD20 with ab194970 at 2.5 μg/mL.

Supplier Data

Western blot - Anti-CD20 antibody - C-terminal (AB194970)

Primary was incubated for 12 hours. Cell lysate prepared in RIPA buffer.

All lanes:

Western blot - Anti-CD20 antibody - C-terminal (ab194970) at 4 µg/mL

All lanes:

Daudi lysate at 20 µg

Predicted band size: 33 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MS4A1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11836

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-2.5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:182 PubMed37533024

2023

Topographic distribution of inflammation factors in a healing aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Basil E Grüter,Gwendoline Canzanella,Joshua Hägler,Jeannine Rey,Stefan Wanderer,Michael von Gunten,José A Galvan,Rainer Grobholz,Hans-Rudolf Widmer,Luca Remonda,Lukas Andereggen,Serge Marbacher

Haematologica 108:2101-2115 PubMed36655432

2023

The immunomodulatory molecule TIGIT is expressed by chronic lymphocytic leukemia cells and contributes to anergy.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Arruga,Marta Rubin,Despoina Papazoglou,Andrea Iannello,Nikolaos Ioannou,Riccardo Moia,Davide Rossi,Gianluca Gaidano,Marta Coscia,Luca Laurenti,Giovanni D'Arena,John N Allan,Richard R Furman,Tiziana Vaisitti,Alan G Ramsay,Silvia Deaglio

International journal of molecular sciences 23: PubMed36233057

2022

CXCR4, CXCR5 and CD44 May Be Involved in Homing of Lymphoma Cells into the Eye in a Patient Derived Xenograft Homing Mouse Model for Primary Vitreoretinal Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Neele Babst,Lisa K Isbell,Felix Rommel,Aysegul Tura,Mahdy Ranjbar,Salvatore Grisanti,Cordula Tschuch,Julia Schueler,Soroush Doostkam,Peter C Reinacher,Justus Duyster,Vinodh Kakkassery,Nikolas von Bubnoff

Frontiers in oncology 10:682 PubMed32528875

2020

The Role of BAFF-R Signaling in the Growth of Primary Central Nervous System Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Zhou,Matthias Mulazzani,Iven-Alex von Mücke-Heim,Sigrid Langer,Wenlong Zhang,Hellen Ishikawa-Ankerhold,Martin Dreyling,Andreas Straube,Louisa von Baumgarten

Cell reports 30:3411-3423.e7 PubMed32160546

2020

Transferrin Receptor Is a Specific Ferroptosis Marker.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhong Feng,Kenji Schorpp,Jenny Jin,Carrie E Yozwiak,Benjamin G Hoffstrom,Aubrianna M Decker,Presha Rajbhandari,Michael E Stokes,Hannah G Bender,Joleen M Csuka,Pavan S Upadhyayula,Peter Canoll,Koji Uchida,Rajesh K Soni,Kamyar Hadian,Brent R Stockwell

Nature communications 10:3705 PubMed31420544

2019

Expression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Serra-Peinado,Judith Grau-Expósito,Laura Luque-Ballesteros,Antonio Astorga-Gamaza,Jordi Navarro,Jenny Gallego-Rodriguez,Mario Martin,Adrià Curran,Joaquin Burgos,Esteban Ribera,Berta Raventós,Rein Willekens,Ariadna Torrella,Bibiana Planas,Rosa Badía,Felipe Garcia,Josep Castellví,Meritxell Genescà,Vicenç Falcó,Maria J Buzon

View all publications

style

left-column

style

left-column

style

right-column