AB215866

Anti-CD20 antibody [IGEL/773]

Name: Anti-CD20 antibody [IGEL/773] (ab215866) | Abcam
Brand: Abcam Limited
SKU: AB215866
Price: 495 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD20 antibody. Suitable for ICC, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MS4A1.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

3 Images

Supplier Data

Immunocytochemistry - Anti-CD20 antibody [IGEL/773] (AB215866)

Immunocytochemistry immunoflourescence image staining Raji cells (human Burkitt's lymphoma cell line) stained with ab215866 (green), nuclear counterstaining with redDot (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] (AB215866)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] (AB215866)

Immunohistochemical analysis of paraffin embedded, formalin fixed Human tonsil tissue labeling CD20 using ab215866 at 1 μg/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] (AB215866)

Immunohistochemical analysis of paraffin embedded, formalin fixed Human lymphoma tissue labeling CD20 using ab215866 at 1 μg/mL dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IGEL/773

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MS4A1.

P11836

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:1846-1858 PubMed36748311

2023

Prognostic relevance of tumor-resident memory T cells in metastatic lymph nodes of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Seiji Natsuki,Hiroaki Tanaka,Masaki Nishiyama,Takuya Mori,Sota Deguchi,Yuichiro Miki,Mami Yoshii,Tatsuro Tamura,Takahiro Toyokawa,Shigeru Lee,Kiyoshi Maeda

Archives of pathology & laboratory medicine 147:167-176 PubMed35687787

2022

Plasma Galectin-9 Is a Useful Biomarker for Predicting Renal Function in Patients Undergoing Native Kidney Biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Tsun Tsai,Ruey-Bing Yang,Shuo-Ming Ou,Wei-Cheng Tseng,Kuo-Hua Lee,Chih-Yu Yang,Fu-Pang Chang,Der-Cherng Tarng

View all publications

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