AB213033

Anti-CD20 antibody [IGEL/773] - BSA and Azide free

Name: Anti-CD20 antibody [IGEL/773] - BSA and Azide free (ab213033) | Abcam
Brand: Abcam Limited
SKU: AB213033
Price: 905 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal CD20 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MS4A1.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] - BSA and Azide free (AB213033)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] - BSA and Azide free (AB213033)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD20 with ab213033 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [IGEL/773] - BSA and Azide free (AB213033)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymphoma tissue labeling CD20 with ab213033 at 1 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IGEL/773

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MS4A1.

P11836

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab213033 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.

Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 2:545-562 PubMed35122017

2021

Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Sheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F Quail

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

Primary Antibodies

AB78237

Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (AB78237)

Primary Antibodies

AB166865

Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (AB166865)

Primary Antibodies

AB198941

Alexa Fluor® 488 Anti-CD20 antibody [EP459Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD20 antibody [EP459Y] (AB198941)

Primary Antibodies

AB198943

Alexa Fluor® 647 Anti-CD20 antibody [EP459Y]

Recombinant|Advanced Validation|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD20 antibody [EP459Y] (AB198943)

Primary Antibodies

AB236434

Anti-CD20 antibody [SP32] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] - BSA and Azide free (AB236434)

Complementary Products

Assay Kits

AB285273

Human CD20 ELISA Kit

Sandwich ELISA - Human CD20 ELISA Kit (AB285273)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

Primary Antibodies

AB20034

Anti-FOXP3 antibody [236A/E7]

BOND RX™ Validated|Recombinant|Lab Essentials|Advanced Validation

Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] (AB20034)

Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com