Anti-CD20 antibody [L26] (ab9475) is a mouse monoclonal antibody that is used to detect CD20 in IHC-P. Suitable for Human samples.
- Over 70 publications
- Trusted since 2002
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (AB9475), expandable thumbnail](https://content.abcam.com/products/images/cd20-antibody-l26-ab9475--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122067.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (AB9475)")
Publications
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- Cancer research and treatment 56:624-6332023Clinical Outcomes of Small Cell Carcinoma of the Genitourinary Tract and the Prognostic Significance of the Tumor Immune Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaewon Hyung,Hyung-Don Kim,Gi Hwan Kim,Yong Mee Cho,Yeon-Mi Ryu,Sang-Yeob Kim,Inkeun Park,Shinkyo Yoon,Jae Lyun LeePubMed 38037320
- Science advances 9:eadi99672023Intracellular delivery of nitric oxide enhances the therapeutic efficacy of mesenchymal stem cells for myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Hao,Guangbo Ji,Meng Qian,Qiu Xuan Li,Haoyan Huang,Shiyu Deng,Pei Liu,Weiliang Deng,Yongzhen Wei,Ju He,Shusen Wang,Wenqing Gao,Tong Li,Jiansong Cheng,Jinwei Tian,Leiting Pan,Fei Gao,Zongjin Li,Qiang ZhaoPubMed 38019911
- Diagnostics (Basel, Switzerland) 13:2023Clinical Usefulness of Immune Profiling for Differential Diagnosis between Crohn's Disease, Intestinal Tuberculosis, and Behcet's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Won Yoo,Su In Jo,Dong Woo Shin,Ji Won Park,Sung-Eun Kim,Hyun Lim,Ho Suk Kang,Sung-Hoon Moon,Min Kyu Kim,Sang-Yeob Kim,Sung Wook Hwang,Jae Seung SohPubMed 37761270
- JTCVS open 15:433-4452023Evaluation of tissue-engineered human acellular vessels as a Blalock-Taussig-Thomas shunt in a juvenile primate model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin M Nash,Brian A Boe,Sergio A Carrillo,Andrew Harrison,Ryuma Iwaki,John Kelly,Robert D Kirkton,Ramkumar Krishnamurthy,Jeffrey H Lawson,Yuichi Matsuzaki,Heather L Prichard,Kejal Shah,Toshiharu Shinoka,Christopher K BreuerPubMed 37808023
- Nature medicine 29:2068-20782023Neoadjuvant adebrelimab in locally advanced resectable esophageal squamous cell carcinoma: a phase 1b trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Yin,Jingnan Yuan,Yunjin Li,Yong Fang,Ruoxi Wang,Heng Jiao,Han Tang,Shaoyuan Zhang,Siyun Lin,Feng Su,Jianmin Gu,Tian Jiang,Dong Lin,Zhiliang Huang,Chaoxiang Du,Kui Wu,Lijie Tan,Qing ZhouPubMed 37488287
- Cancers 15:2023Molecular Subtypes and Tumor Microenvironment Characteristics of Small-Cell Lung Cancer Associated with Platinum-Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihyun Kim,Sunshin Kim,Seog-Yun Park,Geon Kook Lee,Kun Young Lim,Jin Young Kim,Jung-Ah Hwang,Namhee Yu,Eun Hye Kang,Mihwa Hwang,Bo Ram Song,Charny Park,Ji-Youn HanPubMed 37509231
- Frontiers in immunology 14:11727682023Prognostic impact of intra- and peritumoral immune cell subpopulations in head and neck squamous cell carcinomas - comprehensive analysis of the TCGA-HNSC cohort and immunohistochemical validation on 101 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoritz Knebel,Sandrina Körner,Jan Philipp Kühn,Silke Wemmert,Lukas Brust,Sigrun Smola,Mathias Wagner,Rainer M Bohle,Luc G T Morris,Abhinav Pandey,Bernhard Schick,Maximilian LinxweilerPubMed 37383237
- Journal for immunotherapy of cancer 11:2023Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiao He,Qihua He,Xiuyu Cai,Jun Liu,Hongshen Deng,Feng Li,Ran Zhong,Yi Lu,Haoxin Peng,Xiangrong Wu,Zisheng Chen,Shen Lao,Caichen Li,Jianfu Li,Jianxing He,Wenhua LiangPubMed 37072348
- International journal of immunopathology and pharmacology 37:39463202311580252023Infiltrating immune cells in prostate cancer tissue after androgen deprivation and radiotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnn Erlandsson,Marie Lundholm,Johan Watz,Anders Bergh,Elitsa Petrova,Farhood Alamdari,Thomas Helleday,Sabina Davidsson,Ove Andren,Firas TarishPubMed 36880147
- Scientific reports 13:28992023Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshifumi Kida,Toshiya Okahisa,Yasushi Sato,Masahiro Bando,Shota Fujimoto,Beibei Ma,Tadahiko Nakagawa,Tomoyuki Kawaguchi,Fumika Nakamura,Koichi Okamoto,Hiroshi Miyamoto,Masahiro Sogabe,Koichi Tsuneyama,Tetsuji TakayamaPubMed 36806262
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | Flow Cyt | |
|---|---|---|
| Human | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
Alternative names
CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1
Recommended products
Anti-CD20 antibody [L26] (ab9475) is a mouse monoclonal antibody that is used to detect CD20 in IHC-P. Suitable for Human samples.
- Over 70 publications
- Trusted since 2002
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- L26
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD20 antibody [L26] (ab9475) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
Trusted by the scientific community
Anti-CD20 [L26] (ab9475) was first used in a scientific publication in 2002 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-CD20 [L26] (ab9475) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
- Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
- Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
- Associated diseases and disorders
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475), expandable thumbnail](https://content.abcam.com/products/images/cd20-antibody-l26-ab9475--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122067.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)
Formalin-fixed, paraffin-embeded human tonsil tissue stained for CD20 using ab9475 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
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