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AB219329

Anti-CD20 antibody [rIGEL/773]

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(7 Publications)

Mouse Recombinant Monoclonal CD20 antibody. Suitable for ICC, Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MS4A1.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

4 Images
Flow Cytometry - Anti-CD20 antibody [rIGEL/773] (AB219329)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD20 antibody [rIGEL/773] (AB219329)

Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 μg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunocytochemistry - Anti-CD20 antibody [rIGEL/773] (AB219329)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD20 antibody [rIGEL/773] (AB219329)

Immunocytochemistry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 μg/mL followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [rIGEL/773] (AB219329)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [rIGEL/773] (AB219329)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections labeling CD20 with ab219329 at 1 μg/mL.

Protein Array - Anti-CD20 antibody [rIGEL/773] (AB219329)
  • Protein Array

Unknown

Protein Array - Anti-CD20 antibody [rIGEL/773] (AB219329)

ab219329 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

rIGEL/773

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, Protein Array, IHC-P, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MS4A1.

P11836

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:456 PubMed40762017

2025

Acute myeloid leukemia with plasmacytoid dendritic cell proliferation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Pei,Yi Zhang,Han Xu,Pei Pei,Zhengyang Zhang,Hongxia Wang,Bei Zhang,Junjun Bai,Yingxin Zhao,Jingjing Gu,Zhiyu Fang,Miaomiao Liu,Qinglin Song

The European respiratory journal 65: PubMed39401853

2024

Skewed adaptive immune responses are involved in alpha-1 antitrypsin deficiency emphysema.

Applications

Unspecified application

Species

Unspecified reactive species

Joselyn Rojas-Quintero,Scott A Ochsner,Hyun-Sung Lee,Christine Cong,Alan Waich Cohen,Adrianne S Colborg,Konstantin Tsoyi,Maria C Basil,Edward Cantu,Ivan O Rosas,Neil J McKenna,Raúl San-José Estépar,Igor Barjaktarevic,Andrew A Wilson,Francesca Polverino

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

Nature communications 14:8353 PubMed38114474

2023

High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Janesick,Robert Shelansky,Andrew D Gottscho,Florian Wagner,Stephen R Williams,Morgane Rouault,Ghezal Beliakoff,Carolyn A Morrison,Michelli F Oliveira,Jordan T Sicherman,Andrew Kohlway,Jawad Abousoud,Tingsheng Yu Drennon,Seayar H Mohabbat,Sarah E B Taylor

Brain : a journal of neurology 146:1175-1185 PubMed36642091

2023

Haemorrhage of human foetal cortex associated with SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Massimo,Carlotta Barelli,Catalina Moreno,Chiara Collesi,Rebecca K Holloway,Berta Crespo,Lorena Zentilin,Anna Williams,Veronique E Miron,Mauro Giacca,Katherine R Long

Nature biotechnology 41:717-727 PubMed36593395

2023

Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Gravina,Grigol Tediashvili,Raja Rajalingam,Zoe Quandt,Chad Deisenroth,Sonja Schrepfer,Tobias Deuse

Oncology letters 14:7506-7512 PubMed29344196

2017

Downregulated expression of TSHR is associated with distant metastasis in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tianrun Liu,Qianqian Men,Xuan Su,Weichao Chen,Lan Zou,Qiuli Li,Ming Song,Dian Ouyang,Yanfeng Chen,Zhaoqu Li,Xiaoyan Fu,Ankui Yang
View all publications
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Product promise

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