Anti-CD20 antibody [SP32]

23 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

  CD20 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-CD20 antibody

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with ab64088 for 30 mins at room temperature.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with ab64088 for 30 mins at room temperature

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with ab64088 for 30 mins at room temperature.

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  Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] (ab64088)

  Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD20 with purified ab64088 at 1/100 (3.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] (ab64088)

  Flow cytometry analysis of Ramos (human Burkitt's lymphoma) labeling CD20 with purified ab64088 at 1/40 dilution (7.78 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabelled cells (blue).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemical analysis of CD20 expression in formalin fixed, paraffin embedded human tonsil tissue using ab64088 at a 1/100 dilution.

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  Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] (ab64088)

  Flow cytometry analysis of WEHI-231 (mouse lymphoblast B cell lymphoma) labeling CD20 with purified ab64088 at 1/40 dilution (7.78 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabelled cells (blue).

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  Western blot - Anti-CD20 antibody [SP32] (ab64088)

  All lanes: Western blot - Anti-CD20 antibody [SP32] (ab64088) at 1/100 dilution

  All lanes: lysate prepared from Ramos cells

  Predicted band size: 33 kDa

  Observed band size: 33 kDa

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  Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] (ab64088)

  Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody ab64088(1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human palatine tonsil tissue. Stained with ab64088 at 1/100 dilution. Secondary antibody used was Dako EnVision+ System- HRP Labelled Polymer Anti-R. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Goat Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining TCR beta with Anti-TCR beta antibody [RM2056] ab318205 at a 1:150 (0.06 ug/ml) dilution, Anti-TCR beta antibody [RM2056] ab318205 anti-TCR beta used at 1:500 (1.036 ug/ml) dilution and ab64088 anti-CD20 used at a 1:50 (0.2 ug/ml) dilution.

  Panel A: merged staining of anti-CD3 (magenta; Opal™690), anti-TCR beta (green; Opal™520) and anti-CD20 (yellow; Opal™570) on human spleen.
  
  Panel B: anti-CD3 staining T lymphocytes in human spleen.
  
  Panel C: anti-TCR beta staining T lymphocytes in human spleen.
  
  Panel D: anti-CD20 staining B lymphocytes in human spleen.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-CD3 epsilon antibody [SP7], prediluted ab21703, Anti-TCR beta antibody [RM2056] ab318205 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining TCR beta with Anti-TCR beta antibody [RM2056] ab318205 at a 1:150 (0.06 ug/ml) dilution, Anti-TCR beta antibody [RM2056] ab318205 anti-TCR beta used at 1:500 (1.036 ug/ml) dilution and ab64088 anti-CD20 used at a 1:50 (0.2 ug/ml) dilution.

  Panel A: merged staining of anti-CD3 (magenta; Opal™690), anti-TCR beta (green; Opal™520) and anti-CD20 (yellow; Opal™570) on human tonsil.
  
  Panel B: anti-CD3 staining T lymphocytes in human tonsil.
  
  Panel C: anti-TCR beta staining T lymphocytes in human tonsil.
  
  Panel D: anti-CD20 staining B lymphocytes in human tonsil.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-CD3 epsilon antibody [SP7], prediluted ab21703, Anti-TCR beta antibody [RM2056] ab318205 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spleen tissue staining TCR beta with Anti-TCR beta antibody [RM2056] ab318205 at a 1:150 (0.06 ug/ml) dilution, Anti-TCR beta antibody [RM2056] ab318205 anti-TCR beta used at 1:2000 (0.259 ug/ml) dilution and ab64088 anti-CD20 used at a 1:50 (0.2 ug/ml) dilution.

  Panel A: merged staining of anti-CD3 (magenta; Opal™690), anti-TCR beta (green; Opal™520) and anti-CD20 (yellow; Opal™570) on rat spleen.
  
  Panel B: anti-CD3 staining T lymphocytes in rat spleen.
  
  Panel C: anti-TCR beta staining T lymphocytes in rat spleen.
  
  Panel D: anti-CD20 staining B lymphocytes in rat spleen.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-CD3 epsilon antibody [SP7], prediluted ab21703, Anti-TCR beta antibody [RM2056] ab318205 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining TCR beta with Anti-TCR beta antibody [RM2056] ab318205 at a 1:150 (0.06 ug/ml) dilution, Anti-TCR beta antibody [RM2056] ab318205 anti-TCR beta used at 1:2000 (0.259 ug/ml) dilution and ab64088 anti-CD20 used at a 1:50 (0.2 ug/ml) dilution.

  Panel A: merged staining of anti-CD3 (magenta; Opal™690), anti-TCR beta (green; Opal™520) and anti-CD20 (yellow; Opal™570) on mouse spleen.
  
  Panel B: anti-CD3 staining T lymphocytes in mouse spleen.
  
  Panel C: anti-TCR beta staining T lymphocytes in mouse spleen.
  
  Panel D: anti-CD20 staining B lymphocytes in mouse spleen.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-CD3 epsilon antibody [SP7], prediluted ab21703, Anti-TCR beta antibody [RM2056] ab318205 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  CD20 Multiplex immunohistochemistry staining of Rat spleen using rabbit Anti-CD20 antibody

  This image was generated using Anti-CD20 antibody [SP32] - BSA and Azide free ab236434, the same clone, but with a different buffer formulation.
  
  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 at 1:5000 dilution.
  
  Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on rat spleen.
  Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
  Panel C: anti-CD3 epsilon stained on T cells.
  Panel D: anti-CD20 stained on B cells.
  
  The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Western blot - Anti-CD20 antibody [SP32] (ab64088)

  Western blot: Anti-MS4A1 antibody [SP32] (ab64088) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab64088 was shown to bind specifically to MS4A1. A band was observed at 33 kDa in wild-type Raji cell lysates with no signal observed at this size in MS4A1 knockout cell line. To generate this image, wild-type and MS4A1 knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  All lanes: Western blot - Anti-CD20 antibody [SP32] (ab64088) at 1/1000 dilution

  Lane 1: Wild-type Raji cell lysate

  Lane 2: MS4A1 knockout Raji cell lysate

  Lane 2: Western blot - Human MS4A1 (CD20) knockout Raji cell line (Human MS4A1 (CD20) knockout Raji cell line ab273871)

  Lane 3: Ramos cell lysate

  Lane 4: A549 cell lysate

  Secondary

  All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  CD20 Multiplex immunohistochemistry staining of Mouse spleen using rabbit Anti-CD20 antibody

  This image was generated using Anti-CD20 antibody [SP32] - BSA and Azide free ab236434, the same clone, but with a different buffer formulation.
  
  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 at 1:5000 dilution.
  
  Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on mouse spleen.
  Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
  Panel C: anti-CD3 epsilon stained on T cells.
  Panel D: anti-CD20 stained on B cells.
  
  The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  CD20 Multiplex immunohistochemistry staining of rat liver tissue using rabbit Anti-CD20 antibody

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, ab64088 anti-CD20 used at 1:100 (2.91 ug/ml) dilution and Anti-CD3 epsilon antibody [CAL57] ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

  Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD20 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on rat liver.
  
  Panel B: anti-MARCO staining macrophages in rat liver.
  
  Panel C: ant-CD20 staining B lymphocytes in rat liver.
  
  Panel D: ant-CD3 staining T lymphocytes in rat liver.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, ab64088 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) Rat lymph node tissue labelling MAdCAM1 with Anti-MAdCAM1 antibody [EPR27223-58] ab309487 at 1:100 dilution (B), CD3 epsilon with ab237721at 1:2000 dilution (C) and CD20 with ab64088 at 1:100 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-MAdCAM (green; Opal™520), anti-CD3 (gray; Opal™690) and anti-CD20 (magenta; Opal™570) on rat lymph node.

  Panel B: anti-MAdCAM staining high endothelial venules in rat lymph node.

  Panel C: ant-CD3 staining B lymphocytes in rat lymph node.

  Panel D: ant-CD20 staining B lymphocytes in rat lymph node.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-MAdCAM1 antibody [EPR27223-58] ab309487, Anti-CD3 epsilon antibody [CAL57] ab237721 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) Mouse lymph node tissue labelling MAdCAM1 with Anti-MAdCAM1 antibody [EPR27223-58] ab309487 at 1:100 dilution (B), CD3 epsilon with ab237721at 1:2000 dilution (C) and CD20 with ab64088 at 1:100 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-MAdCAM (green; Opal™520), anti-CD3 (gray; Opal™690) and anti-CD20 (magenta; Opal™570) on mouse lymph node.

  Panel B: anti-MAdCAM staining high endothelial venules in mouse lymph node.

  Panel C: ant-CD3 staining B lymphocytes in mouse lymph node.

  Panel D: ant-CD20 staining B lymphocytes in mouse lymph node.

  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-MAdCAM1 antibody [EPR27223-58] ab309487, Anti-CD3 epsilon antibody [CAL57] ab237721 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] (ab64088)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining TCR beta with Anti-TCR beta antibody [RM2056] ab318205 at a 1:150 (0.06 ug/ml) dilution, Anti-TCR beta antibody [RM2056] ab318205 anti-TCR beta used at 1:500 (1.036 ug/ml) dilution and ab64088 anti-CD20 used at a 1:50 (0.2 ug/ml) dilution.

  Panel A: merged staining of anti-CD3 (magenta; Opal™690), anti-TCR beta (green; Opal™520) and anti-CD20 (yellow; Opal™570) on human colon.
  
  Panel B: anti-CD3 staining T lymphocytes in human colon.
  
  Panel C: anti-TCR beta staining T lymphocytes in human colon.
  
  Panel D: anti-CD20 staining B lymphocytes in human colon.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-CD3 epsilon antibody [SP7], prediluted ab21703, Anti-TCR beta antibody [RM2056] ab318205 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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  Immunoprecipitation - Anti-CD20 antibody [SP32] (ab64088)

  ab64088 at 1/30 dilution immunoprecipitating CD20 in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate. Western blot was performed from the immunoprecipitate using ab64088 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
  
  Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
  Lane 2: ab64088 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab64088 in Raji whole cell lysate
  
  
  Blocking/Dilution buffer: 5% NFDM/TBST.
  Exposure time: 8 seconds.

  Lane 2: Immunoprecipitation - Anti-CD20 antibody [SP32] (ab64088) at 1/30 dilution

  Lane 2: Immunoprecipitation - Anti-CD20 antibody [SP32] - BSA and Azide free (Anti-CD20 antibody [SP32] - BSA and Azide free ab236434)

  All lanes: HeLa (human cervix adenocarcinoma epithelial cell) treated with 500 µM CoCl2 for 24 hours whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 25 kDa, 33 kDa

  Exposure time: 8s

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (ab64088)

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed mouse spleen tissue. Stained with ab64088 at 1/100 dilution. Secondary antibody used was Dako EnVision+ System- HRP Labelled Polymer Anti-R. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Goat Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0.