Rabbit Recombinant Monoclonal CD20 antibody. Carrier free. Suitable for IP, mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | mIHC | IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|
Human | Tested | Expected | Tested | Expected | Tested | Tested |
Mouse | Expected | Tested | Expected | Expected | Expected | Tested |
Rat | Expected | Tested | Expected | Expected | Expected | Expected |
Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Select an associated product type
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1, CD20
Rabbit Recombinant Monoclonal CD20 antibody. Carrier free. Suitable for IP, mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1, CD20
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
SP32
Affinity purification Protein A/G
Purified from TCS by protein A/G.
Blue Ice
+4°C
Do Not Freeze
ab236434 is the carrier-free version of Anti-CD20 antibody [SP32] ab64088.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD20 with Anti-CD20 antibody [SP32] ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature This image was generated using Anti-CD20 antibody [SP32] ab64088, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD20 with Anti-CD20 antibody [SP32] ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. This image was generated using Anti-CD20 antibody [SP32] ab64088, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD20 with Anti-CD20 antibody [SP32] ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. This image was generated using Anti-CD20 antibody [SP32] ab64088, the same clone, but with a different buffer formulation.
Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD20 with purified Anti-CD20 antibody [SP32] ab64088 at 1/100 (3.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).
Flow cytometry analysis of Ramos (human Burkitt's lymphoma ) labeling CD20 with purified Anti-CD20 antibody [SP32] ab64088 at 1/40 dilution (7.78 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).
Immunohistochemical analysis of CD20 expression in formalin fixed, paraffin embedded human tonsil tissue using Anti-CD20 antibody [SP32] ab64088 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).
Flow cytometry analysis of WEHI-231 (mouse lymphoblast B cell lymphoma) labeling CD20 with purified Anti-CD20 antibody [SP32] ab64088 at 1/40 dilution (7.78 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).
Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody Anti-CD20 antibody [SP32] ab64088(1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).
Immunohistochemistry analysis of paraformaldehyde fixed Human tonsil tissue staining CD20 with ab236434 at 1/400 and Alexa Fluor 555 donkey anti rabbit secondary. The tissue was incubated with the primary antibody for 16 hours at 4 degrees with PBS + 2% FCS. 5% normal human serum was used for blocking for 1 hour at room temperature.
CD20 immunohistochemistry staining of mouse spleen using rabbit anti-CD20 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and ab236434 at 1:5000 dilution.
Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on mouse spleen.
Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
Panel C: anti-CD3 epsilon stained on T cells.
Panel D: anti-CD20 stained on B cells.
The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Anti-CD20 antibody [SP32] ab64088 at 1/30 dilution immunoprecipitating CD20 in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate. Western blot was performed from the immunoprecipitate using Anti-CD20 antibody [SP32] ab64088 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
Lane 2: Anti-CD20 antibody [SP32] ab64088 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD20 antibody [SP32] ab64088 in Raji whole cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).
Lane 2: Immunoprecipitation - Anti-CD20 antibody [SP32] (Anti-CD20 antibody [SP32] ab64088) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) treated with 500 µM CoCl2 for 24 hours whole cell lysate at 10 µg
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 25 kDa, 33 kDa
Exposure time: 8s
Anti-CD20 antibody [SP32] ab64088 at 1/30 dilution immunoprecipitating CD20 in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate. Western blot was performed from the immunoprecipitate using Anti-CD20 antibody [SP32] ab64088 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
Lane 2: Anti-CD20 antibody [SP32] ab64088 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD20 antibody [SP32] ab64088 in Raji whole cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD20 with ab236434 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 min with ULTRA cell conditioning solution (CC1 pH 8.5). ab236434 anti CD20 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
CD20 immunohistochemistry staining of rat spleen using rabbit anti-CD20 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and ab236434 at 1:5000 dilution.
Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on rat spleen.
Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
Panel C: anti-CD3 epsilon stained on T cells.
Panel D: anti-CD20 stained on B cells.
The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com