Anti-CD20 antibody [SP32] - BSA and Azide free

13 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD20 with Anti-CD20 antibody [SP32] ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature This image was generated using Anti-CD20 antibody [SP32] ab64088, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD20 with Anti-CD20 antibody [SP32] ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. This image was generated using Anti-CD20 antibody [SP32] ab64088, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD20 with Anti-CD20 antibody [SP32] ab64088 at 1/100 dilution (3.11 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. This image was generated using Anti-CD20 antibody [SP32] ab64088, the same clone, but with a different buffer formulation.

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  Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD20 with purified Anti-CD20 antibody [SP32] ab64088 at 1/100 (3.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).

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  Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Flow cytometry analysis of Ramos (human Burkitt's lymphoma ) labeling CD20 with purified Anti-CD20 antibody [SP32] ab64088 at 1/40 dilution (7.78 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunohistochemical analysis of CD20 expression in formalin fixed, paraffin embedded human tonsil tissue using Anti-CD20 antibody [SP32] ab64088 at a 1/100 dilution.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).

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  Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Flow cytometry analysis of WEHI-231 (mouse lymphoblast B cell lymphoma) labeling CD20 with purified Anti-CD20 antibody [SP32] ab64088 at 1/40 dilution (7.78 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).

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  Flow Cytometry (Intracellular) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody Anti-CD20 antibody [SP32] ab64088(1/100)  in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).

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  This image is courtesy of a customer review submitted by Natalie Papazian

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunohistochemistry analysis of paraformaldehyde fixed Human tonsil tissue staining CD20 with ab236434 at 1/400 and Alexa Fluor 555 donkey anti rabbit secondary. The tissue was incubated with the primary antibody for 16 hours at 4 degrees with PBS + 2% FCS. 5% normal human serum was used for blocking for 1 hour at room temperature.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  CD20 immunohistochemistry staining of mouse spleen using rabbit anti-CD20 antibody
  
  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and ab236434 at 1:5000 dilution.
  
  Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on mouse spleen.
  Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
  Panel C: anti-CD3 epsilon stained on T cells.
  Panel D: anti-CD20 stained on B cells.
  
  The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Immunoprecipitation - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Anti-CD20 antibody [SP32] ab64088 at 1/30 dilution immunoprecipitating CD20 in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate. Western blot was performed from the immunoprecipitate using Anti-CD20 antibody [SP32] ab64088 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
  
  Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
  Lane 2: Anti-CD20 antibody [SP32] ab64088 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD20 antibody [SP32] ab64088 in Raji whole cell lysate
  
  Blocking/Dilution buffer: 5% NFDM/TBST.
  Exposure time: 8 seconds.
  
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD20 antibody [SP32] ab64088).

  Lane 2: Immunoprecipitation - Anti-CD20 antibody [SP32] (Anti-CD20 antibody [SP32] ab64088) at 1/30 dilution

  Lane 2: Immunoprecipitation - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  All lanes: HeLa (human cervix adenocarcinoma epithelial cell) treated with 500 µM CoCl2 for 24 hours whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 25 kDa, 33 kDa

  Exposure time: 8s

  Anti-CD20 antibody [SP32] ab64088 at 1/30 dilution immunoprecipitating CD20 in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate. Western blot was performed from the immunoprecipitate using Anti-CD20 antibody [SP32] ab64088 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
  
  Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
  Lane 2: Anti-CD20 antibody [SP32] ab64088 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD20 antibody [SP32] ab64088 in Raji whole cell lysate
  
  
  Blocking/Dilution buffer: 5% NFDM/TBST.
  Exposure time: 8 seconds.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD20 with ab236434 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 min with ULTRA cell conditioning solution (CC1 pH 8.5). ab236434 anti CD20 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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  Multiplex immunohistochemistry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

  CD20 immunohistochemistry staining of rat spleen using rabbit anti-CD20 antibody
  
  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat spleen tissue labeling Sialoadhesin/CD169, CD3 epsilon and CD20 with Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840 at 1/100 dilution, Anti-CD3 epsilon antibody [SP7] ab16669 at 1:150 dilution and ab236434 at 1:5000 dilution.
  
  Panel A: merged staining of anti-Sialoadhesin/CD169 (red; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD20 (gray; Opal™570) on rat spleen.
  Panel B: anti-Sialoadhesin/CD169 stained on macrophages.
  Panel C: anti-CD3 epsilon stained on T cells.
  Panel D: anti-CD20 stained on B cells.
  
  The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.