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AB254213

Anti-CD200 / OX2 antibody [EPR22412-53] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CD200 / OX2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein

1 Images
Flow Cytometry - Anti-CD200 / OX2 antibody [EPR22412-53] - BSA and Azide free (AB254213)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD200 / OX2 antibody [EPR22412-53] - BSA and Azide free (AB254213)

Flow cytometric analysis of 4% paraformaldehyde-fixed human peripheral blood mononuclear cells labeling CD200 at 1/50 dilution (right) compared to a Rabbit monoclonal IgG (ab172730, Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) was used as the secondary antibody at 1/5000 dilution.

Cells were stained with rabbit IgG (Left) or ab252920 (Right). Then stained with anti-CD19 conjugated with Alexa Fluor® 647. The expression pattern is consistent with literature (PMID : 28587354).

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252920).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22412-53

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab254213 is the carrier-free version of ab252920.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.
Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
See full target information CD200
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity 54:1594-1610.e11 PubMed34174183

2021

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Schwabenland,Henrike Salié,Jovan Tanevski,Saskia Killmer,Marilyn Salvat Lago,Alexandra Emilia Schlaak,Lena Mayer,Jakob Matschke,Klaus Püschel,Antonia Fitzek,Benjamin Ondruschka,Henrik E Mei,Tobias Boettler,Christoph Neumann-Haefelin,Maike Hofmann,Angele Breithaupt,Nafiye Genc,Christine Stadelmann,Julio Saez-Rodriguez,Peter Bronsert,Klaus-Peter Knobeloch,Thomas Blank,Robert Thimme,Markus Glatzel,Marco Prinz,Bertram Bengsch
View all publications
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Product promise

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