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Rabbit Recombinant Monoclonal CD200 / OX2 antibody. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CD200 / OX2 antibody [EPR28087-82] (AB314662), expandable thumbnail
  • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (AB314662), expandable thumbnail
  • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (AB314662), expandable thumbnail
  • Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] (AB314662), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (AB314662), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity).

Alternative names

CD200, Mox2, Cd200, OX-2 membrane glycoprotein, MRC OX-2 antigen

Recommended products

Rabbit Recombinant Monoclonal CD200 / OX2 antibody. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28087-82
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.

Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

Associated diseases and disorders

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    CD200 / OX2 Immunocytochemistry/ Immunofluorescence staining of mouse primary neuron using rabbit Anti-CD200 / OX2 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling CD200 / OX2 with ab314662 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
    Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
    Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    CD200 / OX2 Western blot staining using rabbit Anti-CD200 / OX2 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Low expression: liverLysates were freshly made and used for western blotting immediately to minimize protein degradation. In western blot, anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45-50 kDa

    Exposure time: 48s

  • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were freshly made and used for western blotting immediately to minimize protein degradation.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 45-50 kDa

    Exposure time: 103s

  • Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    CD200 / OX2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab314662 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314662 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Mouse brain tissue lysate
    Lane 2: ab314662 IP in Mouse brain tissue lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314662 in mouse brain tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662) at 1/30 dilution

    All lanes: Mouse brain tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 102s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD200 / OX2 with ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Low expression: no staining on hepatocytes, only endothelial cells are stained. The section was incubated with ab314662 for 30 mins at room temperature. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer tissue labeling CD200 / OX2 with ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on endothelium of mouse pancreatic cancer. The section was incubated with ab314662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CD200 / OX2 with ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on mouse cerebrum. The section was incubated with ab314662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] (ab314662)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD200 / OX2 with ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on mouse spleen. The section was incubated with ab314662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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