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Rabbit Recombinant Monoclonal CD200 / OX2 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse samples.

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Images

Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (AB314663), expandable thumbnail
  • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (AB314663), expandable thumbnail
  • Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (AB314663), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (AB314663), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (AB314663), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

    Target data

    Function

    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity).

    Alternative names

    OX-2 membrane glycoprotein, MRC OX-2 antigen, Mox2, Cd200

    Recommended products

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    Rabbit Recombinant Monoclonal CD200 / OX2 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF and reacts with Mouse samples.

    Alternative names

    OX-2 membrane glycoprotein, MRC OX-2 antigen, Mox2, Cd200

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR28087-82

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab314663 is the carrier-free version of Anti-CD200 / OX2 antibody [EPR28087-82] ab314662.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.

    Biological function summary

    CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

    Pathways

    CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

    Associated diseases and disorders

    CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    8 product images

    • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST.
      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      All lanes: Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (Anti-CD200 / OX2 antibody [EPR28087-82] ab314662) at 1/1000 dilution

      Lane 1: Mouse testis tissue lysate at 20 µg

      Lane 2: Mouse spleen tissue lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 45-50 kDa

      Exposure time: 103s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.
      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    • Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: 5% NFDM/TBST.
      Low expression: liverLysates were freshly made and used for western blotting immediately to minimize protein degradation. In western blot, anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

      All lanes: Western blot - Anti-CD200 / OX2 antibody [EPR28087-82] (Anti-CD200 / OX2 antibody [EPR28087-82] ab314662) at 1/1000 dilution

      Lane 1: Mouse brain tissue lysate at 20 µg

      Lane 2: Mouse liver tissue lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 45-50 kDa

      Exposure time: 48s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.
      Low expression: liverLysates were freshly made and used for western blotting immediately to minimize protein degradation. In western blot, anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    • Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      CD200 / OX2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
      Lane 1: Mouse brain tissue lysate
      Lane 2: Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 IP in Mouse brain tissue lysate
      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 in mouse brain tissue lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      All lanes: Immunoprecipitation - Anti-CD200 / OX2 antibody [EPR28087-82] (Anti-CD200 / OX2 antibody [EPR28087-82] ab314662) at 1/30 dilution

      All lanes: Mouse brain tissue lysate

      Secondary

      All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

      Exposure time: 102s

      CD200 / OX2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
      Lane 1: Mouse brain tissue lysate
      Lane 2: Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 IP in Mouse brain tissue lysate
      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 in mouse brain tissue lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
      Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
      Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Low expression: no staining on hepatocytes, only endothelial cells are stained. The section was incubated with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 for 30 mins at room temperature. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer tissue labeling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Positive staining on endothelium of mouse pancreatic cancer. The section was incubated with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Positive staining on mouse cerebrum. The section was incubated with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR28087-82] - BSA and Azide free (ab314663)

      This data was developed using Anti-CD200 / OX2 antibody [EPR28087-82] ab314662, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD200 / OX2 with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Positive staining on mouse spleen. The section was incubated with Anti-CD200 / OX2 antibody [EPR28087-82] ab314662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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