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AB22353

Anti-CD200 / OX2 antibody [MRC OX-2]

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(2 Publications)

Mouse Monoclonal CD200 / OX2 antibody. Suitable for Flow Cyt and reacts with Rat samples. Cited in 2 publications.

View Alternative Names

CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein

1 Images
Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (AB22353)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (AB22353)

Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

  • 578 PE

    PE Anti-CD200 / OX2 antibody [MRC OX-2]

  • Carrier free

    Anti-CD200 / OX2 antibody [MRC OX-2] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

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Primary Antibodies

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Secondary Antibodies

AB150077

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Primary Antibodies

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Anti-PD-L1 antibody [28-8]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

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Primary Antibodies

AB229021

Anti-CD134 / OX40L receptor antibody [EPR22229-5]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (AB229021)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.
Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
See full target information CD200
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brain research 1481:90-6 PubMed22975132

2012

Expression of CD200 in alternative activation of microglia following an excitotoxic lesion in the mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Hee Yi,Enji Zhang,Joon Won Kang,Yu Na Shin,Jin Young Byun,Sang-Ha Oh,Je Hoon Seo,Young Ho Lee,Dong Woon Kim

The Journal of neuroscience : the official journal of the Society for Neuroscience 27:8309-13 PubMed17670977

2007

CD200 ligand receptor interaction modulates microglial activation in vivo and in vitro: a role for IL-4.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Lyons,Eric J Downer,Suzanne Crotty,Yvonne M Nolan,Kingston H G Mills,Marina A Lynch
View all publications
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Product promise

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