AB33734

Anti-CD200 / OX2 antibody [MRC OX90]

Name: Anti-CD200 / OX2 antibody [MRC OX90] (ab33734) | Abcam
Brand: Abcam Limited
SKU: AB33734
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rat Monoclonal CD200 / OX2 antibody. Suitable for IHC-FoFr and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Protein within Mouse Cd200.

View Alternative Names

CD200, Mox2, Cd200, OX-2 membrane glycoprotein, MRC OX-2 antigen

1 Images

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CD200 / OX2 antibody [MRC OX90] (AB33734)

IHC-FoFr

AbReview32638****

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CD200 / OX2 antibody [MRC OX90] (AB33734)

Immunohistochemical analysis of murine brain tissue, staining CD200 / OX2 with ab33734.

Tissue was fixed with paraformaldehyde and blocked with 5% BSA + 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/600 in 5% BSA + 5% NGS) for 17 hours at 4°C. An AlexaFluor®555-conjugated goat anti-rat polyclonal IgG (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

519 FITC

FITC Anti-CD200 / OX2 antibody [MRC OX90]
578 PE

PE Anti-CD200 / OX2 antibody [MRC OX90]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MRC OX90

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

IHC-FoFr

applications

Immunogen

Recombinant Protein within Mouse Cd200. The exact immunogen used to generate this antibody is proprietary information.

O54901

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>" } } }

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Product details

Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This purified antibody was prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.

Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity).

See full target information Cd200

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature biomedical engineering 8:1285-1307 PubMed39085645

2024

Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Vincentius Jeremy Suhardi,Anastasia Oktarina,Mohammed Hammad,Yingzhen Niu,Qingdian Li,Andrew Thomson,Juan Lopez,Jason McCormick,Ugur M Ayturk,Matthew B Greenblatt,Lionel B Ivashkiv,Mathias P G Bostrom,Xu Yang

Brain and behavior 10:e01882 PubMed33067924

2020

CD200-CD200R1 signaling pathway regulates neuroinflammation after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Cai Zhao,Xu Heng,Wang Ya-Ping,Luan Di,Wu Wen-Qian,Ma Ling-Song,Zhao-Hu Chu,Yang Xu

Nature 562:133-139 PubMed30250253

2018

Discovery of a periosteal stem cell mediating intramembranous bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Shawon Debnath,Alisha R Yallowitz,Jason McCormick,Sarfaraz Lalani,Tuo Zhang,Ren Xu,Na Li,Yifang Liu,Yeon Suk Yang,Mark Eiseman,Jae-Hyuck Shim,Meera Hameed,John H Healey,Mathias P Bostrom,Dan Avi Landau,Matthew B Greenblatt

Brain, behavior, and immunity 26:778-88 PubMed21986303

2011

Age-related changes in the hippocampus (loss of synaptophysin and glial-synaptic interaction) are modified by systemic treatment with an NCAM-derived peptide, FGL.

Applications

Unspecified application

Species

Unspecified reactive species

Bunmi Ojo,Payam Rezaie,Paul L Gabbott,Heather Davies,Frances Colyer,Thelma R Cowley,Marina Lynch,Michael G Stewart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD200 / OX2 antibody [MRC OX90]

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CD200 / OX2 antibody [MRC OX90] (AB33734)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise