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AB244564

Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free

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(1 Publication)

Rat Monoclonal CD200 / OX2 antibody. Carrier free. Suitable for Flow Cyt, ELISA, IHC-FoFr, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD200, Mox2, Cd200, OX-2 membrane glycoprotein, MRC OX-2 antigen

1 Images
Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (AB244564)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (AB244564)

This data was developed using the same antibody clone in a different buffer formulation (ab33734)

C57BL/6 mouse splenocytes stained with ab33734 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33734) or Rat IgG2aκ (ab18450) (1x106 in 100 μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD19 antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

  • 519 FITC

    FITC Anti-CD200 / OX2 antibody [MRC OX90]

  • 578 PE

    PE Anti-CD200 / OX2 antibody [MRC OX90]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MRC OX90

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-FoFr, ELISA, Flow Cyt, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

ab244564 is the carrier-free version of ab33734.

Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.
Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity).
See full target information Cd200
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunology 102:173-9 PubMed11260322

2001

The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.

Applications

Unspecified application

Species

Unspecified reactive species

G J Wright,M Jones,M J Puklavec,M H Brown,A N Barclay
View all publications
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Product promise

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