AB23552

Anti-CD200 / OX2 antibody [OX-104]

Name: Anti-CD200 / OX2 antibody [OX-104] (ab23552) | Abcam
Brand: Abcam Limited
SKU: AB23552
Price: 470 USD
Availability: InStock

(1 Review)

(4 Publications )

Mouse Monoclonal CD200 / OX2 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications.

1 Images

Flow Cytometry - Anti-CD200 / OX2 antibody [OX-104] (AB23552)

Flow Cyt

Lab

Flow Cytometry - Anti-CD200 / OX2 antibody [OX-104] (AB23552)

Human peripheral blood mononuclear cells stained with ab23552 (right) or mouse IgG1 kappa (ab170190) isotype (left). Human peripheral blood mononuclear cells were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab23552) or mouse IgG1 kappa (ab170190) (1x10⁶ in 100 μl at 1 μg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor^® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 APC antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

Carrier free

Anti-CD200 / OX2 antibody [OX-104] - BSA and Azide free
660 APC

APC Anti-CD200 / OX2 antibody [OX-104]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104]
519 FITC

FITC Anti-CD200 / OX2 antibody [OX-104]
578 PE

PE Anti-CD200 / OX2 antibody [OX-104]
778 PE/Cy7®

PE/Cy7® Anti-CD200 / OX2 antibody [OX-104]
387 mFluor™ UV375

mFluor™ UV375 Anti-CD200 antibody [OX-104]
454 mFluor™ Violet 450

mFluor™ Violet 450 Anti-CD200 antibody [OX-104]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-104

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.

Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

See full target information CD200

Alternative Names

CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein

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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Stem cell research & therapy 10:155 PubMed31151466

2019

Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Huateng Zhou,Lixiang Wang,Cui Zhang,Jintao Hu,Jianlin Chen,Weibin Du,Fei Liu,Weifan Ren,Jinfu Wang,Renfu Quan

Scientific reports 6:29047 PubMed27381832

2016

3D map of the human corneal endothelial cell.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguo He,Fabien Forest,Philippe Gain,Damien Rageade,Aurélien Bernard,Sophie Acquart,Michel Peoc'h,Dennis M Defoe,Gilles Thuret

PloS one 8:e84324 PubMed24376802

2013

Identification of a population of epidermal squamous cell carcinoma cells with enhanced potential for tumor formation.

Applications

Unspecified application

Species

Human

Gautam Adhikary,Dan Grun,Candace Kerr,Sivaprakasam Balasubramanian,Ellen A Rorke,Mohan Vemuri,Shayne Boucher,Jackie R Bickenbach,Thomas Hornyak,Wen Xu,Matthew L Fisher,Richard L Eckert

PloS one 7:e46087 PubMed23049945

2012

Activation of protease-activated receptor 2 induces VEGF independently of HIF-1.

Applications

Flow Cyt

Species

Human

Jeppe Grøndahl Rasmussen,Simone Elkjær Riis,Ole Frøbert,Sufang Yang,Jens Kastrup,Vladimir Zachar,Ulf Simonsen,Trine Fink

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD200 / OX2 antibody [OX-104]

Flow Cytometry - Anti-CD200 / OX2 antibody [OX-104] (AB23552)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (4)

Product promise