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AB217843

Anti-CD204 antibody

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(4 Publications)

Rabbit Polyclonal CD204 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MSR1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD204, SCARA1, MSR1, Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (AB217843)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (AB217843)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human glioma tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (AB217843)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody (AB217843)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human esophageal cancer tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-CD204 antibody (AB217843)
  • WB

Supplier Data

Western blot - Anti-CD204 antibody (AB217843)

12% gel

All lanes:

Western blot - Anti-CD204 antibody (ab217843) at 1/200 dilution

Lane 1:

Human colon carcinoma lysates

Lane 2:

rat brain lysates

Secondary

All lanes:

goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution

Predicted band size: 50 kDa

Observed band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MSR1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P21757

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD204 also known as MSR1 or the macrophage scavenger receptor 1 is a class A scavenger receptor. It has a molecular mass of approximately 80-85 kDa. CD204 is mainly expressed on the surface of macrophages as well as some dendritic cells. This receptor plays a role in mediating the endocytosis of various polyanionic molecules such as modified low-density lipoproteins. CD204 serves as a recognition site for apoptotic cells signaling molecules and some types of bacteria.
Biological function summary

CD204 influences inflammatory responses and lipid metabolism. It does not function alone but as part of a complex involving several other molecules like pattern recognition receptors. CD204 recognizes and binds to modified lipoproteins and is significant in processes like phagocytosis and clearance of cellular debris. Its activity affects immune response modulation and can influence atherosclerosis development by its interaction with oxidized low-density lipoproteins.

Pathways

The role of CD204 extends to key cellular and immunological pathways. This receptor is involved in the Toll-like receptor signaling pathway which is important for innate immunity besides participating in the macrophage activation pathways. In these pathways CD204 interacts with other proteins including CD36 and SRA. This interaction helps coordinate inflammatory responses and the resolution of inflammation.

CD204 is associated with atherosclerosis and chronic inflammation. Its connection with these conditions arises from its role in lipid uptake and immune cell regulation within the vascular system. In the context of atherosclerosis CD204's interaction with Apolipoprotein E (ApoE) influences the formation of foam cells which are significant in the disease's progression. Similarly in chronic inflammation CD204 modulates macrophage behavior impacting the inflammatory environment and potentially affecting the disease outcome.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL) (PubMed : 2251254). Isoform III does not internalize acetylated LDL (PubMed : 9548586).
See full target information MSR1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 44:411-428 PubMed39169784

2024

Unveiling the Role of Sik1 in Osteoblast Differentiation: Implications for Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kuanmin Tian,Xiaoxin He,Xue Lin,Xiaolei Chen,Yajing Su,Zhidong Lu,Zhirong Chen,Liang Zhang,Peng Li,Long Ma,Zhibin Lan,Xin Zhao,Gangning Fen,Qinqin Hai,Di Xue,Qunhua Jin

International journal of molecular sciences 22: PubMed34502382

2021

Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuki Kai,Masafumi Moriyama,A S M Rafiul Haque,Taichi Hattori,Akira Chinju,Chen Hu,Keigo Kubota,Yuka Miyahara,Noriko Kakizoe-Ishiguro,Shintaro Kawano,Seiji Nakamura

The Journal of biological chemistry 297:100948 PubMed34252459

2021

Recognition of lipoproteins by scavenger receptor class A members.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cheng,Enlin Zheng,Bowen Yu,Ze Zhang,Yuanyuan Wang,Yingbin Liu,Yongning He

The Journal of biological chemistry 294:18881-18897 PubMed31653705

2019

The scavenger receptor SCARA1 (CD204) recognizes dead cells through spectrin.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cheng,Zhenzheng Hu,Longxing Cao,Chao Peng,Yongning He
View all publications
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