Anti-CD204 antibody [EPR24403-17] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling CD204 with ab271070 at 1/50 (10.32 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing increased cytoplasmic staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (80 nM) for 72 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Kupffer cells in human liver (PMID : 28202073). The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on macrophages in human lung. The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells treated with 80nM phorbol 12-myristate 13-acetate (PMA) for 72 hours (Right) / Untreated control (Left), labelling CD204 with ab271070 at 1/50 dilution (1ug). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in human hepatocellular carcinoma (PMID : 28202073). The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling CD204 with ab271070 at 1/100 (5.16 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in human gastric carcinoma (PMID : 28202073). The section was incubated with ab271070 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation.

CD204 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte), treated with 80nM PMA for 72 hours, whole cell lysate with ab271070 at 1/30 dilution (2 ug in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab271070 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : THP-1(human monocytic leukemia monocyte) treated with 80nM PMA for 72 hours whole cell lysate 20 ug

Lane 2 : ab271070 IP in THP-1 treated with 80nM PMA for 72 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271070 in THP-1 treated with 80nM PMA for 72 hours whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 6 seconds.

All lanes:

Immunoprecipitation - Anti-CD204 antibody [EPR24403-17] (<a href='/en-us/products/primary-antibodies/cd204-antibody-epr24403-17-ab271070'>ab271070</a>)

Predicted band size: 50 kDa

Observed band size: 80 kDa

false

• WB

Lab

Western blot - Anti-CD204 antibody [EPR24403-17] - BSA and Azide free (AB283512)

This data was developed using ab271070, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30991038). Exposure time : Lanes 1-2 : 26 seconds; Lanes 3-4 : 8 seconds.

All lanes:

Western blot - Anti-CD204 antibody [EPR24403-17] (<a href='/en-us/products/primary-antibodies/cd204-antibody-epr24403-17-ab271070'>ab271070</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 4:

THP-1 treated with 80nM PMA for 72 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 50 kDa

Observed band size: 80 kDa

false