Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)

Rabbit Recombinant Monoclonal CD204 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Mouse samples.

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Images

Western blot - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (AB314228), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt	IP
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Msr1

Function

Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL).

Alternative names

CD204, Scvr, Msr1, Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor type A, SR-A

Recommended products

Rabbit Recombinant Monoclonal CD204 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28270-88
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD204 also known as MSR1 or the macrophage scavenger receptor 1 is a class A scavenger receptor. It has a molecular mass of approximately 80-85 kDa. CD204 is mainly expressed on the surface of macrophages as well as some dendritic cells. This receptor plays a role in mediating the endocytosis of various polyanionic molecules such as modified low-density lipoproteins. CD204 serves as a recognition site for apoptotic cells signaling molecules and some types of bacteria.

Biological function summary

CD204 influences inflammatory responses and lipid metabolism. It does not function alone but as part of a complex involving several other molecules like pattern recognition receptors. CD204 recognizes and binds to modified lipoproteins and is significant in processes like phagocytosis and clearance of cellular debris. Its activity affects immune response modulation and can influence atherosclerosis development by its interaction with oxidized low-density lipoproteins.

Pathways

The role of CD204 extends to key cellular and immunological pathways. This receptor is involved in the Toll-like receptor signaling pathway which is important for innate immunity besides participating in the macrophage activation pathways. In these pathways CD204 interacts with other proteins including CD36 and SRA. This interaction helps coordinate inflammatory responses and the resolution of inflammation.

Associated diseases and disorders

CD204 is associated with atherosclerosis and chronic inflammation. Its connection with these conditions arises from its role in lipid uptake and immune cell regulation within the vascular system. In the context of atherosclerosis CD204's interaction with Apolipoprotein E (ApoE) influences the formation of foam cells which are significant in the disease's progression. Similarly in chronic inflammation CD204 modulates macrophage behavior impacting the inflammatory environment and potentially affecting the disease outcome.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Neuro-2a, NIH/3T3.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35937296).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 5.5 seconds; Lane 2-4: 37 seconds.
All lanes: Western blot - Anti-CD204 antibody [EPR28270-88] (Anti-CD204 antibody [EPR28270-88] ab314227) at 1/1000 dilution
Lane 1: J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg
Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell pellets; (B) Neuro-2a (mouse neuroblastoma neuroblast) cell pellets. tissue labeling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell pellets, no staining on (B) Neuro-2a (mouse neuroblastoma neuroblast) cell pellets.The section was incubated with Anti-CD204 antibody [EPR28270-88] ab314227 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse neuroendocrine carcinoma tissue labeling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in mouse neuroendocrine carcinoma.The section was incubated with Anti-CD204 antibody [EPR28270-88] ab314227 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in mouse lung cancer.The section was incubated with Anti-CD204 antibody [EPR28270-88] ab314227 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells in mouse colon.The section was incubated with Anti-CD204 antibody [EPR28270-88] ab314227 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on macrophages in mouse lung.The section was incubated with Anti-CD204 antibody [EPR28270-88] ab314227 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/50 (10.26 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse bone marrow treated with lipopolysaccharide (2 ug/mL) for 24 h.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-F4/80 rat monoclonal antibody was used to counterstain tubulin at 1/100 10 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/50 (10.26 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line.Negative control: Neuro-2a Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Isotype (Left) / Mouse bone marrow treated with 2ug/ml LPS for 24h (Middle) / Untreated mouse bone marrow (Right) cells labelling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with F4/80 conjugated with Alexa Fluor®647. Gated on viable cells.
Flow Cytometry - Anti-CD204 antibody [EPR28270-88] - BSA and Azide free (ab314228)
This data was developed using Anti-CD204 antibody [EPR28270-88] ab314227, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / Raw264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD204 with Anti-CD204 antibody [EPR28270-88] ab314227 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: Neuro-2a.