AB151707

Anti-CD204 antibody [EPR7536]

RabMAb
Recombinant
20ul selling size
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(12 Publications)

Rabbit Recombinant Monoclonal CD204 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

View Alternative Names

CD204, SCARA1, MSR1, Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1

4 Images

Lab

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (ab151707) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (ab151707) at 1/1000 dilution

All lanes:

Mouse placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (ab151707) at 1/5000 dilution

All lanes:

Rat placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Unknown

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (ab151707) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

THP1 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 50 kDa,79 kDa

false

Carrier free

Anti-CD204 antibody [EPR7536] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7536

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD204 also known as MSR1 or the macrophage scavenger receptor 1 is a class A scavenger receptor. It has a molecular mass of approximately 80-85 kDa. CD204 is mainly expressed on the surface of macrophages as well as some dendritic cells. This receptor plays a role in mediating the endocytosis of various polyanionic molecules such as modified low-density lipoproteins. CD204 serves as a recognition site for apoptotic cells signaling molecules and some types of bacteria.

Biological function summary

CD204 influences inflammatory responses and lipid metabolism. It does not function alone but as part of a complex involving several other molecules like pattern recognition receptors. CD204 recognizes and binds to modified lipoproteins and is significant in processes like phagocytosis and clearance of cellular debris. Its activity affects immune response modulation and can influence atherosclerosis development by its interaction with oxidized low-density lipoproteins.

Pathways

The role of CD204 extends to key cellular and immunological pathways. This receptor is involved in the Toll-like receptor signaling pathway which is important for innate immunity besides participating in the macrophage activation pathways. In these pathways CD204 interacts with other proteins including CD36 and SRA. This interaction helps coordinate inflammatory responses and the resolution of inflammation.

CD204 is associated with atherosclerosis and chronic inflammation. Its connection with these conditions arises from its role in lipid uptake and immune cell regulation within the vascular system. In the context of atherosclerosis CD204's interaction with Apolipoprotein E (ApoE) influences the formation of foam cells which are significant in the disease's progression. Similarly in chronic inflammation CD204 modulates macrophage behavior impacting the inflammatory environment and potentially affecting the disease outcome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL) (PubMed : 2251254). Isoform III does not internalize acetylated LDL (PubMed : 9548586).

See full target information MSR1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:542 PubMed37607939

2023

Smooth muscle liver kinase B1 inhibits foam cell formation and atherosclerosis via direct phosphorylation and activation of SIRT6.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Deng,Hongxuan Li,Xiaolin Yue,Chenghu Guo,Yuanyuan Sun,Chang Ma,Jiangang Gao,Yue Wu,Bin Du,Jianmin Yang,Cheng Zhang,Wencheng Zhang

EBioMedicine 90:104480 PubMed36863256

2023

A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sites.

Applications

Unspecified application

Species

Unspecified reactive species

Yawei Wang,Binlin Tang,Huijuan Li,Jiancheng Zheng,Can Zhang,Zeyu Yang,Xu Tan,Peng Luo,Le Ma,Yang Wang,Lei Long,Zelin Chen,Zhenliang Xiao,Lijie Ma,Jing Zhou,Yu Wang,Chunmeng Shi

International journal of molecular sciences 24: PubMed36768908

2023

Activation of the RARα Attenuated CSF Hypersecretion to Inhibit Hydrocephalus Development via Regulating the MAFB/MSR1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hanhai Zeng,Kaibo Yu,Junyou Wang,Jingya Ye,Huaijun Chen,Chaoran Xu,Ting Chen,Feng Yan,Gao Chen,Chi Gu

Nature communications 13:6804 PubMed36357401

2022

Ferritin triggers neutrophil extracellular trap-mediated cytokine storm through Msr1 contributing to adult-onset Still's disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinchao Jia,Mengyan Wang,Jianfen Meng,Yuning Ma,Yang Wang,Naijun Miao,Jialin Teng,Dehao Zhu,Hui Shi,Yue Sun,Honglei Liu,Xiaobing Cheng,Yutong Su,Junna Ye,Huihui Chi,Tingting Liu,Zhuochao Zhou,Liyan Wan,Xia Chen,Fan Wang,Hao Zhang,Jingjing Ben,Jing Wang,Chengde Yang,Qiongyi Hu

Frontiers in cell and developmental biology 8:609090 PubMed33490072

2021

Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Jun Lv,Hao Wang,Hong-Yong Cui,Ze-Kun Liu,Ren-Yu Zhang,Meng Lu,Can Li,Yu-Le Yong,Man Liu,Hai Zhang,Tian-Jiao Zhang,Kun Zhang,Gang Li,Gang Nan,Cong Zhang,Shuang-Ping Guo,Ling Wang,Zhi-Nan Chen,Huijie Bian

Nutrients 12: PubMed33261070

2020

Phenethyl Isothiocyanate Protects against High Fat/Cholesterol Diet-Induced Obesity and Atherosclerosis in C57BL/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Hee Gwon,Young-Sun Im,A-Reum Seo,Kyoung Yun Kim,Ha-Rin Moon,Jung-Mi Yun

Journal of cardiovascular translational research : PubMed32285315

2020

Deficient Chaperone-Mediated Autophagy Promotes Lipid Accumulation in Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Qiao,He-Feng Wang,Lei Xiang,Jing Ma,Qiang Zhu,Dan Xu,Hui Zheng,Jie-Qiong Peng,Sen Zhang,Hui-Xia Lu,Wen-Qiang Chen,Yun Zhang

The EMBO journal 38: PubMed31028084

2019

Triggering MSR1 promotes JNK-mediated inflammation in IL-4-activated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Manman Guo,Anetta Härtlova,Marek Gierliński,Alan Prescott,Josep Castellvi,Javier Hernandez Losa,Sine K Petersen,Ulf A Wenzel,Brian D Dill,Christoph H Emmerich,Santiago Ramon Y Cajal,David G Russell,Matthias Trost

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:2290-2300 PubMed30222079

2018

CTRP13 inhibits atherosclerosis via autophagy-lysosome-dependent degradation of CD36.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Wenjing Xu,Minglu Liang,Dan Huang,Kai Huang

Cardiovascular research 115:395-408 PubMed30169632

2018

Exogenous CXCL4 infusion inhibits macrophage phagocytosis by limiting CD36 signalling to enhance post-myocardial infarction cardiac dilation and mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Merry L Lindsey,Mira Jung,Andriy Yabluchanskiy,Presley L Cannon,Rugmani Padmanabhan Iyer,Elizabeth R Flynn,Kristine Y DeLeon-Pennell,Fritz M Valerio,Courtney L Harrison,Crystal M Ripplinger,Michael E Hall,Yonggang Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD204 antibody [EPR7536]

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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