AB240122

Anti-CD204 antibody [EPR7536] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD204 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CD204, SCARA1, MSR1, Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1

4 Images

Unknown

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/en-us/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

THP1 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 50 kDa,79 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/en-us/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/en-us/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/1000 dilution

All lanes:

Mouse placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/en-us/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/5000 dilution

All lanes:

Rat placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Unconjugated

Anti-CD204 antibody [EPR7536]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7536

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240122 is the carrier-free version of ab151707.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD204 also known as MSR1 or the macrophage scavenger receptor 1 is a class A scavenger receptor. It has a molecular mass of approximately 80-85 kDa. CD204 is mainly expressed on the surface of macrophages as well as some dendritic cells. This receptor plays a role in mediating the endocytosis of various polyanionic molecules such as modified low-density lipoproteins. CD204 serves as a recognition site for apoptotic cells signaling molecules and some types of bacteria.

Biological function summary

CD204 influences inflammatory responses and lipid metabolism. It does not function alone but as part of a complex involving several other molecules like pattern recognition receptors. CD204 recognizes and binds to modified lipoproteins and is significant in processes like phagocytosis and clearance of cellular debris. Its activity affects immune response modulation and can influence atherosclerosis development by its interaction with oxidized low-density lipoproteins.

Pathways

The role of CD204 extends to key cellular and immunological pathways. This receptor is involved in the Toll-like receptor signaling pathway which is important for innate immunity besides participating in the macrophage activation pathways. In these pathways CD204 interacts with other proteins including CD36 and SRA. This interaction helps coordinate inflammatory responses and the resolution of inflammation.

CD204 is associated with atherosclerosis and chronic inflammation. Its connection with these conditions arises from its role in lipid uptake and immune cell regulation within the vascular system. In the context of atherosclerosis CD204's interaction with Apolipoprotein E (ApoE) influences the formation of foam cells which are significant in the disease's progression. Similarly in chronic inflammation CD204 modulates macrophage behavior impacting the inflammatory environment and potentially affecting the disease outcome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL) (PubMed : 2251254). Isoform III does not internalize acetylated LDL (PubMed : 9548586).

See full target information MSR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:5323-5335 PubMed32606750

2020

Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting β-Tubulin in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Fu,Xiaoyin Sun,Zhangyuan Gu,Zhigang Zhuang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD204 antibody [EPR7536] - BSA and Azide free

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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