Name: Anti-CD208 antibody [EPR24265-8] - BSA and Azide free
SKU: ab281573
Rating: 5 (3 reviews)

Rabbit Recombinant Monoclonal CD208 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	Flow Cyt (Intra)
Human	Not recommended	Not recommended	Not recommended	Tested	Not recommended
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information LAMP3

Function

Lysosomal membrane glycoprotein which plays a role in the unfolded protein response (UPR) that contributes to protein degradation and cell survival during proteasomal dysfunction (PubMed:25681212). Plays a role in the process of fusion of the lysosome with the autophagosome, thereby modulating the autophagic process (PubMed:24434718). Promotes hepatocellular lipogenesis through activation of the PI3K/Akt pathway (PubMed:29056532). May also play a role in dendritic cell function and in adaptive immunity (PubMed:9768752). (Microbial infection) Plays a positive role in post-entry steps of influenza A virus replication, either virus uncoating, cytosolic transport, or nuclear import of viral components, and promotes nuclear accumulation of influenza nucleoprotein/NP at early stages of viral infection. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. (Microbial infection) Promotes the intracellular proliferation of Salmonella typhimuium.

Alternative names

CD208, DCLAMP, TSC403, LAMP3, Lysosome-associated membrane glycoprotein 3, LAMP-3, Lysosomal-associated membrane protein 3, DC-lysosome-associated membrane glycoprotein, Protein TSC403, DC LAMP

Recommended products

Rabbit Recombinant Monoclonal CD208 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24265-8
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab281573 is the carrier-free version of Anti-CD208 antibody [EPR24265-8] ab271053.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein CD208 also known as DC-LAMP or LAMP-3 is integral to the immune system. It has an approximate mass of 60 kDa. CD208 is a type I transmembrane glycoprotein found mainly in the endosomal/lysosomal compartments of dendritic cells. The expression of CD208 is upregulated during the maturation of dendritic cells making it a reliable marker for these immune cells. Researchers often use CD208 to study dendritic cell biology and its role in immune responses.

Biological function summary

CD208 participates in the regulation of the immune response by facilitating antigen processing and presentation. This protein is a part of the lysosomal-associated membrane protein family which contributes to the recycling of MHC class II molecules. Through its involvement in the endocytic pathway CD208 aids in presenting antigens to T lymphocytes an important step in triggering adaptive immunity. The protein colocalizes with MHC class II which is essential for effective antigen presentation.

Pathways

CD208 has a significant role in the endosomal-lysosomal pathway where it interacts with other proteins involved in antigen processing. It also engages in the intracellular trafficking pathway that affects the presentation of peptides on MHC class II molecules. The interaction with proteins such as HLA-DR is vital for efficient antigen presentation to helper T cells. CD208 supports the proper delivery of peptide-loaded MHC molecules to the cell surface a necessity for a functional adaptive immune response.

Associated diseases and disorders

CD208 has implications in autoimmune diseases and infectious diseases. Its expression is notably altered in conditions like lupus and certain infectious diseases. In systemic lupus erythematosus (SLE) abnormal CD208 expression correlates with the dysregulation of immune responses. Additionally during infectious diseases changes in CD208 expression can impact the activation and function of dendritic cells affecting disease progression. The interaction of CD208 with proteins like MHC class II molecules highlights its role in disease pathology and immune regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
This data was developed using Anti-CD208 antibody [EPR24265-8] ab271053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD208 with Anti-CD208 antibody [EPR24265-8] ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Dendritic cells in human spleen (PMID: 23528943).The section was incubated with Anti-CD208 antibody [EPR24265-8] ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
This data was developed using Anti-CD208 antibody [EPR24265-8] ab271053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD208 with Anti-CD208 antibody [EPR24265-8] ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Dendritic cells in human tonsil (PMID: 23528943).The section was incubated with Anti-CD208 antibody [EPR24265-8] ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
This data was developed using Anti-CD208 antibody [EPR24265-8] ab271053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling CD208 with Anti-CD208 antibody [EPR24265-8] ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cervical carcinoma.The section was incubated with Anti-CD208 antibody [EPR24265-8] ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
This data was developed using Anti-CD208 antibody [EPR24265-8] ab271053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling CD208 with Anti-CD208 antibody [EPR24265-8] ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cerebrum.The section was incubated with Anti-CD208 antibody [EPR24265-8] ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
This data was developed using Anti-CD208 antibody [EPR24265-8] ab271053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling CD208 with Anti-CD208 antibody [EPR24265-8] ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cardiac muscle.The section was incubated with Anti-CD208 antibody [EPR24265-8] ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.
Panel B: anti-CD208 staining dendritic cells in human tonsil.
Panel C: anti-CD3E staining T lymphocytes in human tonsil.
Panel D: anti-CD19 staining B lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.
Panel B: anti-CD208 staining dendritic cells in human liver cancer.
Panel C: anti-CD3E staining T lymphocytes in human liver cancer.
Panel D: anti-CD19 staining B lymphocytes of human liver cancer.
The section was incubated in three rounds of staining: in the order ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.
Panel B: anti-CD208 staining dendritic cells in human colon cancer.
Panel C: anti-CD3E staining T lymphocytes in human colon cancer.
Panel D: anti-CD19 staining B lymphocytes of human colon cancer.
The section was incubated in three rounds of staining: in the order ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.
Panel B: anti-CD208 staining dendritic cells in human colon.
Panel C: anti-CD3E staining T lymphocytes in human colon.
Panel D: anti-CD19 staining B lymphocytes of human colon.
The section was incubated in three rounds of staining: in the order ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
This image is courtesy of a customer review submitted by Dylan Windell
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (ab281573)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed 0.3% Triton X-100 permeabilized human tonsil tissue staining with ab281573 at 5 µg/ml dilution. Samples were incubated with the primary antibody with for 1 hour at 20°C. Blocking was done using Donkey Serum 10% + 3% BSA for 24 hours at 4°C. Heat mediated antigen retrieval with Citrate pH 6 & TRIS pH 9.