Anti-CD208 antibody [EPR24265-8] - BSA and Azide free

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human thymus tissue staining CD208 with ab324381 at a 1 : 100 (4.94 ug/ml) dilution, ab281573 anti-LAMP3/CD208 used at 1 : 500 (0.938 ug/ml) dilution and ab237772 anti-CD19 used at a 1 : 5000 (0.21 ug/ml) dilution.

Panel A : merged staining of anti-LAMP3/CD208 (green; Opal™520), anti-LAMP3/CD208 (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on human thymus.
Panel B : anti-LAMP3/CD208 staining dendritic cells in human thymus.
Panel C : anti-LAMP3/CD208 staining dendritic cells in human thymus.
Panel D : anti-CD19 staining B lymphocytes of human thymus.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324381, ab271053, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining CD208 with ab324381 at a 1 : 100 (4.94 ug/ml) dilution, ab281573 anti-LAMP3/CD208 used at 1 : 500 (0.938 ug/ml) dilution and ab237772 anti-CD19 used at a 1 : 5000 (0.21 ug/ml) dilution.

Panel A : merged staining of anti-LAMP3/CD208 (green; Opal™520), anti-LAMP3/CD208 (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on human tonsil.
Panel B : anti-LAMP3/CD208 staining dendritic cells in human tonsil.
Panel C : anti-LAMP3/CD208 staining dendritic cells in human tonsil.
Panel D : anti-CD19 staining B lymphocytes of human tonsil.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324381, ab271053, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

This data was developed using ab271053, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human cardiac muscle.The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

This data was developed using ab271053, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human cerebrum.The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

This data was developed using ab271053, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Dendritic cells in human tonsil (PMID : 23528943).The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

This data was developed using ab271053, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Dendritic cells in human spleen (PMID : 23528943).The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

This data was developed using ab271053, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cervical carcinoma.The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.

Panel B : anti-CD208 staining dendritic cells in human liver cancer.

Panel C : anti-CD3E staining T lymphocytes in human liver cancer.

Panel D : anti-CD19 staining B lymphocytes of human liver cancer.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.
Panel B : anti-CD208 staining dendritic cells in human tonsil.
Panel C : anti-CD3E staining T lymphocytes in human tonsil.
Panel D : anti-CD19 staining B lymphocytes of human tonsil.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.

Panel B : anti-CD208 staining dendritic cells in human colon.

Panel C : anti-CD3E staining T lymphocytes in human colon.

Panel D : anti-CD19 staining B lymphocytes of human colon.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.

Panel B : anti-CD208 staining dendritic cells in human colon cancer.

Panel C : anti-CD3E staining T lymphocytes in human colon cancer.

Panel D : anti-CD19 staining B lymphocytes of human colon cancer.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

AbReview80635****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] - BSA and Azide free (AB281573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed 0.3% Triton X-100 permeabilized human tonsil tissue staining with ab281573 at 5 µg/ml dilution. Samples were incubated with the primary antibody with for 1 hour at 20°C. Blocking was done using Donkey Serum 10% + 3% BSA for 24 hours at 4°C. Heat mediated antigen retrieval with Citrate pH 6 & TRIS pH 9.

This image is courtesy of an Abreview submitted by Dylan Windell