Rat Monoclonal C209A antibody. Suitable for Flow Cyt, IHC-Fr, BL and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Cd209a protein.
Images
![Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (AB37220), expandable thumbnail](https://content.abcam.com/products/images/cd209b-dc-signr1-antibody-er-tr9-ab37220--flow-cytometry-img80071.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (AB37220)")
Publications
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- Journal of immunology (Baltimore, Md. : 1950) 200:775-7872017Altered Marginal Zone B Cell Selection in the Absence of IκBNS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Ádori,Gabriel K Pedersen,Csaba Ádori,Elina Erikson,Sharesta Khoenkhoen,Julian M Stark,Jin Huk Choi,Pia Dosenovic,Mikael C I Karlsson,Bruce Beutler,Gunilla B Karlsson HedestamPubMed 29222168
- Journal of immunology (Baltimore, Md. : 1950) 172:1157-622004The role of SIGNR1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages.Applications:Flow Cyt, BLReactive species:MousePhilip R Taylor,Gordon D Brown,Jurgen Herre,David L Williams,Janet A Willment,Siamon GordonPubMed 14707091
- Journal of immunology (Baltimore, Md. : 1950) 171:6495-5012003The selection of marginal zone B cells differs from that of B-1a cells.Applications:IHC-FrReactive species:MouseKarsten Kretschmer,Anke Jungebloud,Jana Stopkowicz,Tanja Kleinke,Reinhard Hoffmann,Siegfried WeissPubMed 14662849
- Immunology 55:23-301985Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno- and enzyme-histochemical properties and functional capacities.Applications:IHC-FrReactive species:MouseC D Dijkstra,E Van Vliet,E A Döpp,A A van der Lelij,G KraalPubMed 3888828
Key facts
- Isotype
- IgM
- Host species
- Rat
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tissue culture supernatant- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing Cd209a protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q91ZX1
Reactivity data
Select an application| Flow Cyt | IHC-Fr | BL | |
|---|---|---|---|
| Mouse | Tested | Expected | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg for 106 Cells | Notes Rat IgM, kappa monoclonal [RTK2118] - Isotype Control ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
BL
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Dilutions have to be made according to the amounts of SIGN-R1 to be inactivated. |
Target data
Function
Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens.
Additional Targets
Cd209b, Cd209d, CD209 antigen-like protein E, Cd209c
Alternative names
CD209, Cire, Cd209a, CD209 antigen-like protein A, Dendritic cell-specific ICAM-3-grabbing non-integrin, DC-SIGN
Recommended products
Rat Monoclonal C209A antibody. Suitable for Flow Cyt, IHC-Fr, BL and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Cd209a protein.
Key facts
- Isotype
- IgM
- Host species
- Rat
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tissue culture supernatant- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing Cd209a protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q91ZX1
- Clone number
- ER-TR9
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD209B also known as DC-SIGNR1 is a C-type lectin receptor with a molecular mass of about 45 kDa. This receptor is similar in structure to CD209A another member of the same gene family. CD209B is mainly expressed on macrophages and dendritic cells which are critical components of the immune system. This receptor plays a role in the immune response by recognizing and binding to specific carbohydrate structures on the surface of various pathogens.
- Biological function summary
CD209B functions as part of a broader set of protein interactions involved in pathogen recognition and clearance. It acts as a pattern recognition receptor and is involved in initiating immune responses. Although not part of a large complex CD209B shares functional similarities with other receptors like CD209A mediating defensive strategies against infections through binding to glycoprotein antigens on microbes.
- Pathways
The receptor participates in the immune signaling networks that oversee pathogen recognition. It is vital within the innate immune pathway where it helps to stimulate adaptive immunity. CD209B engages with proteins such as Toll-like receptors which amplify immune responses against pathogens.
- Associated diseases and disorders
CD209B has potential implications in infectious diseases and autoimmune disorders. Its ability to bind diverse pathogens makes it an important player in infections like HIV where it helps the virus gain entry into host cells. Additionally its interaction with proteins like mannose receptor suggests a role in diseases characterized by chronic inflammation. Understanding these interactions assists in developing interventions to modulate immune response in such conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (ab37220), expandable thumbnail](https://content.abcam.com/products/images/cd209b-dc-signr1-antibody-er-tr9-ab37220--flow-cytometry-img80071.jpg "Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (ab37220)")
Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (ab37220)
Overlay histogram showing SP0/2 cells stained with ab37220 (red line). The cells were fixed with 4% paraformaldehyde (10min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37220, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgM, kappa monoclonal [RTK2118] (Rat IgM, kappa monoclonal [RTK2118] - Isotype Control ab35768, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SP0/2 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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