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AB37220

Anti-CD209B/DC-SIGNR1 antibody [ER-TR9]

5

(2 Reviews)

|

(5 Publications)

Rat Monoclonal C209A antibody. Suitable for Flow Cyt, IHC-Fr, BL and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing Cd209a protein.

View Alternative Names

CD209, Cire, Cd209a, CD209 antigen-like protein A, Dendritic cell-specific ICAM-3-grabbing non-integrin, DC-SIGN

1 Images
Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (AB37220)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD209B/DC-SIGNR1 antibody [ER-TR9] (AB37220)

Overlay histogram showing SP0/2 cells stained with ab37220 (red line). The cells were fixed with 4% paraformaldehyde (10min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37220, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgM, kappa monoclonal [RTK2118] (ab35768, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SP0/2 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

ER-TR9

Isotype

IgM

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr, Flow Cyt, BL

applications

Immunogen

Cell preparation containing Cd209a protein. The exact immunogen used to generate this antibody is proprietary information.

Q91ZX1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "BL" : {"fullname" : "Blocking", "shortname":"BL"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igm-kappa-monoclonal-rtk2118-isotype-control-ab35768'>ab35768</a> - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "BL-species-checked": "guaranteed", "BL-species-dilution-info": "", "BL-species-notes": "<p>Dilutions have to be made according to the amounts of SIGN-R1 to be inactivated.</p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.02% Sodium azide Constituents: Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD209B also known as DC-SIGNR1 is a C-type lectin receptor with a molecular mass of about 45 kDa. This receptor is similar in structure to CD209A another member of the same gene family. CD209B is mainly expressed on macrophages and dendritic cells which are critical components of the immune system. This receptor plays a role in the immune response by recognizing and binding to specific carbohydrate structures on the surface of various pathogens.
Biological function summary

CD209B functions as part of a broader set of protein interactions involved in pathogen recognition and clearance. It acts as a pattern recognition receptor and is involved in initiating immune responses. Although not part of a large complex CD209B shares functional similarities with other receptors like CD209A mediating defensive strategies against infections through binding to glycoprotein antigens on microbes.

Pathways

The receptor participates in the immune signaling networks that oversee pathogen recognition. It is vital within the innate immune pathway where it helps to stimulate adaptive immunity. CD209B engages with proteins such as Toll-like receptors which amplify immune responses against pathogens.

CD209B has potential implications in infectious diseases and autoimmune disorders. Its ability to bind diverse pathogens makes it an important player in infections like HIV where it helps the virus gain entry into host cells. Additionally its interaction with proteins like mannose receptor suggests a role in diseases characterized by chronic inflammation. Understanding these interactions assists in developing interventions to modulate immune response in such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens.
See full target information Cd209a

Additional targets

Cd209b,,CD209 antigen-like protein E,Cd209c
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 200:775-787 PubMed29222168

2017

Altered Marginal Zone B Cell Selection in the Absence of IκBNS.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Ádori,Gabriel K Pedersen,Csaba Ádori,Elina Erikson,Sharesta Khoenkhoen,Julian M Stark,Jin Huk Choi,Pia Dosenovic,Mikael C I Karlsson,Bruce Beutler,Gunilla B Karlsson Hedestam

Journal of virology 89:5904-18 PubMed25810544

2015

CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Qin,Yinyan Yin,Qinghua Yu,Lulu Huang,Xiaoqing Wang,Jian Lin,Qian Yang

Journal of immunology (Baltimore, Md. : 1950) 172:1157-62 PubMed14707091

2004

The role of SIGNR1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages.

Applications

Flow Cyt, BL

Species

Mouse, Mouse

Philip R Taylor,Gordon D Brown,Jurgen Herre,David L Williams,Janet A Willment,Siamon Gordon

Journal of immunology (Baltimore, Md. : 1950) 171:6495-501 PubMed14662849

2003

The selection of marginal zone B cells differs from that of B-1a cells.

Applications

IHC-Fr

Species

Mouse

Karsten Kretschmer,Anke Jungebloud,Jana Stopkowicz,Tanja Kleinke,Reinhard Hoffmann,Siegfried Weiss

Immunology 55:23-30 PubMed3888828

1985

Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno- and enzyme-histochemical properties and functional capacities.

Applications

IHC-Fr

Species

Mouse

C D Dijkstra,E Van Vliet,E A Döpp,A A van der Lelij,G Kraal
View all publications
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