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AB237981

Anti-CD21 antibody [CR2/1953]

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(2 Publications)

Mouse Monoclonal CD21 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CR2 aa 1-200.

View Alternative Names

CD21, C3DR, CR2, Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [CR2/1953] (AB237981)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [CR2/1953] (AB237981)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 with ab237981 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [CR2/1953] (AB237981)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [CR2/1953] (AB237981)

Formalin-fixed, paraffin-embedded human follicular dendritic cell sarcoma tissue stained for CD21 with ab237981 at 2 μg/ml in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CR2/1953

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CR2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P20023

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab237981 is purified from bioreactor concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.
Biological function summary

Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.

Pathways

CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.

CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed : 1849076, PubMed : 21527715, PubMed : 7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed : 21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed : 1383329, PubMed : 30107486). Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed : 12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed : 1386409).. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
See full target information CR2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 52:753-772 PubMed40688064

2025

Modular assembled biomimetic nanobubbles for synergistic therapy of ischemic stroke cascade modulation thrombo-inflammatory network.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxi Li,Xuan Xu,Guoyan Tang,Huan Wu,Yakun Wang,Chen Cheng,Hongde Liu,Fang Yang

The Journal of clinical investigation 132: PubMed34813502

2021

Positive and negative selection shape the human naive B cell repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Jeff W Chen,Jean-Nicolas Schickel,Nikolaos Tsakiris,Joel Sng,Florent Arbogast,Delphine Bouis,Daniele Parisi,Ruchi Gera,Joshua M Boeckers,Fabien R Delmotte,Margaret Veselits,Catharina Schuetz,Eva-Maria Jacobsen,Carsten Posovszky,Ansgar S Schulz,Klaus Schwarz,Marcus R Clark,Laurence Menard,Eric Meffre
View all publications
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