Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)

Rabbit Recombinant Monoclonal CD21 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF, WB, IP, mIHC and reacts with Human, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (AB315161), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	ICC/IF	WB	IP	mIHC
Human	Tested	Tested	Tested	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Human	Not recommended	Not recommended	Not recommended	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information CR2

Function

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed:1849076, PubMed:21527715, PubMed:7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed:21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed:12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed:1386409). (Microbial infection) Acts as a receptor for Epstein-Barr virus.

Alternative names

CD21, C3DR, CR2, Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor

Recommended products

Rabbit Recombinant Monoclonal CD21 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF, WB, IP, mIHC and reacts with Human, Transfected cell lysate - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR27369-9
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315161 is the carrier-free version of Anti-CD21 antibody [EPR27369-9] ab315160.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.

Biological function summary

Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.

Pathways

CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.

Associated diseases and disorders

CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Western blot - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-CD21 antibody [EPR27369-9] (Anti-CD21 antibody [EPR27369-9] ab315160) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: 293T cells transfected with a human CD21 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa
Exposure time: 3.25s
Multiplex immunohistochemistry - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at a 1:50 (10.56 ug/ml) dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 anti-CD19 used at 1:5000 (0.21 ug/ml) dilution and Anti-CD3 epsilon antibody [CAL54] ab237707 anti-CD3E used at a 1:500 (1.01 ug/ml) dilution.
Panel A: merged staining of anti-CD21 (green; Opal™690), anti-CD3E (grey; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.

Panel B: anti-CD21 staining mature B lymphocytes and T-lymphocytes of human tonsil.

Panel C: anti-CD19 staining B lymphocytes of human tonsil.

Panel D: anti-CD3E staining T lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order of Anti-CD21 antibody [EPR27369-9] ab315160, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, and Anti-CD3 epsilon antibody [CAL54] ab237707 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells. Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
Flow Cytometry - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells. Cells are co-stained with CD19 conjugated to Alexa Fluor®647.
Flow Cytometry - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: K-562. Gated on viable cells.
Immunoprecipitation - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
CD21 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD21 antibody [EPR27369-9] ab315160 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2: Anti-CD21 antibody [EPR27369-9] ab315160 IP in Raji (human Burkitt's lymphoma B lymphocyte)
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD21 antibody [EPR27369-9] ab315160 in Raji whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-CD21 antibody [EPR27369-9] (Anti-CD21 antibody [EPR27369-9] ab315160) at 1/30 dilution
All lanes: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 41s
Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/50 (10.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing membranous staining in subsets of human PBMCs. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/50 (10.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing membranous and cytoplasmic staining in Raji cell line. Negative control: K-562 (PMID: 6983911).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/50 (10.56 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human cardiac muscle. The section was incubated with Anti-CD21 antibody [EPR27369-9] ab315160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hodgkin's lymphoma tissue labeling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/50 (10.56 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human hodgkin's lymphoma. The section was incubated with Anti-CD21 antibody [EPR27369-9] ab315160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at 1/50 (10.56 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on germinal center of human tonsil. The section was incubated with Anti-CD21 antibody [EPR27369-9] ab315160 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Human heart.
The identity of the lower MW bands at approximately 50kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 15 seconds Lane 2: 180 seconds
All lanes: Western blot - Anti-CD21 antibody [EPR27369-9] (Anti-CD21 antibody [EPR27369-9] ab315160) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa, 36 kDa
Western blot - Anti-CD21 antibody [EPR27369-9] - BSA and Azide free (ab315161)
This data was developed using Anti-CD21 antibody [EPR27369-9] ab315160, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: K-562 (PMID: 6983911).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 48 seconds Lane 2: 180 seconds
All lanes: Western blot - Anti-CD21 antibody [EPR27369-9] (Anti-CD21 antibody [EPR27369-9] ab315160) at 1/1000 dilution
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa, 36 kDa