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Rabbit Recombinant Monoclonal CD21 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra), WB, IHC-Fr and reacts with Rat, Human, Mouse samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)WBIHC-Fr
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Expected
Not recommended
Tested
Rat
Tested
Tested
Expected
Expected

Tested
Tested

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info

1/100

Notes

-

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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    3 products for Alternative Version

      Target data

      Function

      Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).(Microbial infection) Acts as a receptor for Epstein-Barr virus.

      Alternative names

      Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, C3DR, CR2

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      Rabbit Recombinant Monoclonal CD21 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra), WB, IHC-Fr and reacts with Rat, Human, Mouse samples.

      Alternative names

      Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, C3DR, CR2

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      SP186

      Purification technique

      Affinity purification Protein A/G

      Concentration
      Loading...
      Purification notes

      Purified from TCS by protein A/G.

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      Supplementary info

      Activity summary

      CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.

      Biological function summary

      Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.

      Pathways

      CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.

      Associated diseases and disorders

      CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      16 product images

      • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:20 dilution (5.3 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on mouse cardiac muscle. The section was incubated with ab227662 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on mouse liver. The section was incubated with ab227662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat spleen. The section was incubated with ab227662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

      • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227662 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
        The section was incubated with ab227662 for 10 mins at room temperature.

      • Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        All lanes: Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662) at 1/400 dilution

        All lanes: Daudi (Human Burkitt's lymphoma cell line) cell lysate

        Predicted band size: 113 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen. The section was incubated with ab227662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins

      • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Flow Cytometry analysis of 2.4G2 (rat B cell lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:200 dilution (0.53 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
        The section was incubated with ab227662 for 10 mins at room temperature.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
        The section was incubated with ab227662 for 10 mins at room temperature.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Frozen sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution (Green). membranous staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

        Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 μg/ml dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Formalin-fixed, paraffin-embedded human spleen tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

        Formalin-fixed, paraffin-embedded human colon tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com