Anti-CD21 antibody [SP186] - C-terminal (ab227662) is a rabbit monoclonal antibody that is used to detect CD21 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--flow-cytometry-intracellular-img25243.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img179272.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171454.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168941.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (AB227662)")
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--flow-cytometry-intracellular-img122243.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Expected | Expected | Not recommended | Tested |
| Rat | Tested | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed:1849076, PubMed:21527715, PubMed:7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed:21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed:12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed:1386409). (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Alternative names
CD21, C3DR, CR2, Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor
Recommended products
Anti-CD21 antibody [SP186] - C-terminal (ab227662) is a rabbit monoclonal antibody that is used to detect CD21 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP186
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD21 antibody [SP186] - C-terminal (ab227662) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of CD21?
Anti-CD21 [SP186] - C-terminal (ab227662) specifically detects a band for CD21 (UniProt: P20023) at a molecular weight of 113kDa.
Collaboration
Anti-CD21 [SP186] - C-terminal (ab227662) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [SP186] also available for your convenience:
ab227662, Carrier free - Anti-CD21 antibody [SP186] - BSA and Azide free ab240987, ab279465, PE - PE Anti-CD21 antibody [SP186] (C-terminal) ab306325, APC - APC Anti-CD21 antibody [SP186] (C-terminal) ab306326, HRP - HRP Anti-CD21 antibody [SP186] (C-terminal) ab306327, Alkaline Phosphatase - Alkaline Phosphatase Anti-CD21 antibody [SP186] - C-terminal ab308986, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-CD21 antibody [SP186] - C-terminal ab309929, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CD21 antibody [SP186] - C-terminal ab310296, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-CD21 antibody [SP186] - C-terminal ab310758, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-CD21 antibody [SP186] - C-terminal ab312285, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-CD21 antibody [SP186] - C-terminal ab312780, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-CD21 antibody [SP186] - C-terminal ab321441
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.
- Biological function summary
Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.
- Pathways
CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.
- Associated diseases and disorders
CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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16 product images
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--flow-cytometry-intracellular-img25243.jpg "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:20 dilution (5.3 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img179272.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on mouse cardiac muscle. The section was incubated with ab227662 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171454.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on mouse liver. The section was incubated with ab227662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168941.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat spleen. The section was incubated with ab227662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--flow-cytometry-intracellular-img122243.jpg "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227662 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8918.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227662 for 10 mins at room temperature.![Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--western-blot-img83004.jpg "Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
All lanes: Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662) at 1/400 dilution
All lanes: Daudi (Human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 113 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174202.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen. The section was incubated with ab227662 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--flow-cytometry-intracellular-img137871.jpg "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Flow Cytometry analysis of 2.4G2 (rat B cell lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:200 dilution (0.53 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img51119.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227662 for 10 mins at room temperature.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8924.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227662 for 10 mins at room temperature.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137803.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Frozen sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-frozen-sections-img184655.jpg "Immunohistochemistry (Frozen sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Frozen sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD21 with ab227662 at 1/100 (0.28 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution (Green). membranous staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 μg/ml dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28081.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100564.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Formalin-fixed, paraffin-embedded human spleen tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12205.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
Formalin-fixed, paraffin-embedded human colon tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8918.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
![Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--western-blot-img83004.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174202.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp186-c-terminal-ab227662--flow-cytometry-intracellular-img137871.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)")
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