Rabbit Recombinant Monoclonal CD21 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: 98.9% PBS, 1% BSA
Liquid
Monoclonal
Flow Cyt (Intra) | WB | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/150 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
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Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).(Microbial infection) Acts as a receptor for Epstein-Barr virus.
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, C3DR, CR2
Rabbit Recombinant Monoclonal CD21 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, C3DR, CR2
IgG
Rabbit
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: 98.9% PBS, 1% BSA
Liquid
Monoclonal
SP199
Affinity purification Protein A/G
Purified from TCS by protein A/G.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This supplementary information is collated from multiple sources and compiled automatically.
CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.
Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.
CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.
CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte)labeling CD21 with purified ab227668 at 1/20 dilution (5.1 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227668 at 1/100 dilution (1.02 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227668 for 10 mins at room temperature.
Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227668 at 1/100 (green) compared with a rabbit isotype control (blue).
All lanes: Western blot - Anti-CD21 antibody [SP199] (ab227668) at 1/150 dilution
All lanes: Daudi (human Burkitt's lymphoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 113 kDa
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 with ab227668 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 with ab227668 at 1/100 dilution in immunohistochemical analysis.
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