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Rabbit Recombinant Monoclonal CD21 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] (AB227668), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (AB227668), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] (AB227668), expandable thumbnail
  • Western blot - Anti-CD21 antibody [SP199] (AB227668), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (AB227668), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: 98.9% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-P
Human
Tested
Tested
Tested
Mouse
Predicted
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/20 - 1/100

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/150

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).(Microbial infection) Acts as a receptor for Epstein-Barr virus.

Alternative names

Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, C3DR, CR2

Recommended products

Rabbit Recombinant Monoclonal CD21 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

Alternative names

Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, C3DR, CR2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP199

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.

Biological function summary

Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.

Pathways

CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.

Associated diseases and disorders

CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] (ab227668), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] (ab227668)

    Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte）labeling CD21 with purified ab227668 at 1/20 dilution (5.1 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (ab227668), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (ab227668)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227668 at 1/100 dilution (1.02 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227668 for 10 mins at room temperature.

  • Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] (ab227668), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] (ab227668)

    Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227668 at 1/100 (green) compared with a rabbit isotype control (blue).

  • Western blot - Anti-CD21 antibody [SP199] (ab227668), expandable thumbnail

    Western blot - Anti-CD21 antibody [SP199] (ab227668)

    All lanes: Western blot - Anti-CD21 antibody [SP199] (ab227668) at 1/150 dilution

    All lanes: Daudi (human Burkitt's lymphoma cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 113 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (ab227668), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (ab227668)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 with ab227668 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (ab227668), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] (ab227668)

    Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 with ab227668 at 1/100 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com