Rabbit Recombinant Monoclonal CD21 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106168.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988)")
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--flow-cytometry-intracellular-img122248.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14594.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110603.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (AB240988)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
8 products for Alternative Version
Target data
Function
Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed:1849076, PubMed:21527715, PubMed:7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed:21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed:12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed:1386409). (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Alternative names
CD21, C3DR, CR2, Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor
Recommended products
Rabbit Recombinant Monoclonal CD21 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP199
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240988 is the carrier-free version of Anti-CD21 antibody [SP199] ab227668.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.
- Biological function summary
Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.
- Pathways
CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.
- Associated diseases and disorders
CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106168.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with Anti-CD21 antibody [SP199] ab227668 at 1/100 dilution (1.02 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD21 antibody [SP199] ab227668 for 10 mins at room temperature.This image was generated using Anti-CD21 antibody [SP199] ab227668, the same clone, but with a different buffer formulation.
![Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--flow-cytometry-intracellular-img122248.jpg "Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)")
Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)
Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with Anti-CD21 antibody [SP199] ab227668 at 1/100 (green) compared with a rabbit isotype control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD21 antibody [SP199] ab227668).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14594.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 with Anti-CD21 antibody [SP199] ab227668 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD21 antibody [SP199] ab227668).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988), expandable thumbnail](https://content.abcam.com/products/images/cd21-antibody-sp199-bsa-and-azide-free-ab240988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110603.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)
Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 with Anti-CD21 antibody [SP199] ab227668 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD21 antibody [SP199] ab227668).
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