AB243317

Anti-CD21+CD35 antibody [4B2]

Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Mouse Recombinant Monoclonal CD21 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435μg/ml, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

Negative control : EL4 (PMID : 10453011).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435μg/ml, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-CD21+CD35 antibody [4B2] (AB243317)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Negative control : EL4 (PMID : 10453011).

All lanes:

Western blot - Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/mL

Lane 1:

EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

Lane 2:

2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

Predicted band size: 113 kDa

Observed band size: 155 kDa

false

Carrier free

Anti-CD21+CD35 antibody [4B2] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2.435 µg/mL", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2.435 µg/mL", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.487 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CD21+CD35 target commonly known as CD21/CD35 includes two surface proteins: CD21 and CD35. CD21 also known as complement receptor 2 (CR2) has an approximate mass of 145 kDa. CD35 also called complement receptor 1 (CR1) varies in mass between 190 to 280 kDa due to glycosylation. These proteins are expressed on the surface of B cells dendritic cells and some epithelial cells. Both markers participate in the immune system aiding in the clearance of pathogens and foreign antigens.

Biological function summary

CD21 and CD35 mediate the binding and clearance of opsonized antigens. These proteins form part of larger complexes where they act as receptors for complement proteins like C3d and C4b. CD21 enhances B cell activation by bridging the B-cell receptor with C3d-bound antigens. Meanwhile CD35 helps clear immune complexes reducing inflammation and facilitating healing.

Pathways

CD21/CD35 plays a significant role in the complement cascade and immune complex clearance pathways. Both CD21 and CD35 connect to other complement proteins such as C3. CD21 also interacts with proteins like CD19 and CD81 forming part of the B-cell co-receptor complex which amplifies B-cell receptor signaling and assists in immune response regulation.

CD21/CD35 involvement links with autoimmune conditions such as systemic lupus erythematosus and chronic lymphocytic leukemia. In these diseases defective or altered CD21/CD35 expression can lead to inadequate immune complex clearance or abnormal B cell activation. Dysfunction in this system may alter interactions with proteins like CD19 contributing to disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.

See full target information Cr2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular immunology 63:479-88 PubMed25457881

2014

A new mouse anti-mouse complement receptor type 2 and 1 (CR2/CR1) monoclonal antibody as a tool to study receptor involvement in chronic models of immune responses and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Liudmila Kulik,Finnegan B Hewitt,Van C Willis,Rosa Rodriguez,Stephen Tomlinson,V Michael Holers

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD21+CD35 antibody [4B2]

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317)

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317)

Western blot - Anti-CD21+CD35 antibody [4B2] (AB243317)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise