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Mouse Recombinant Monoclonal CD21 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (AB243317), expandable thumbnail
  • Western blot - Anti-CD21+CD35 antibody [4B2] (AB243317), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PIHC-FrFlow CytIP
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
2.435 µg/mL
Notes

-

Species
Rat
Dilution info
2.435 µg/mL
Notes

-

Tested
Tested

Species
Rat
Dilution info
0.487 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.

Alternative names

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

Recommended products

Mouse Recombinant Monoclonal CD21 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
4B2
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The CD21+CD35 target commonly known as CD21/CD35 includes two surface proteins: CD21 and CD35. CD21 also known as complement receptor 2 (CR2) has an approximate mass of 145 kDa. CD35 also called complement receptor 1 (CR1) varies in mass between 190 to 280 kDa due to glycosylation. These proteins are expressed on the surface of B cells dendritic cells and some epithelial cells. Both markers participate in the immune system aiding in the clearance of pathogens and foreign antigens.

Biological function summary

CD21 and CD35 mediate the binding and clearance of opsonized antigens. These proteins form part of larger complexes where they act as receptors for complement proteins like C3d and C4b. CD21 enhances B cell activation by bridging the B-cell receptor with C3d-bound antigens. Meanwhile CD35 helps clear immune complexes reducing inflammation and facilitating healing.

Pathways

CD21/CD35 plays a significant role in the complement cascade and immune complex clearance pathways. Both CD21 and CD35 connect to other complement proteins such as C3. CD21 also interacts with proteins like CD19 and CD81 forming part of the B-cell co-receptor complex which amplifies B-cell receptor signaling and assists in immune response regulation.

Associated diseases and disorders

CD21/CD35 involvement links with autoimmune conditions such as systemic lupus erythematosus and chronic lymphocytic leukemia. In these diseases defective or altered CD21/CD35 expression can lead to inadequate immune complex clearance or abnormal B cell activation. Dysfunction in this system may alter interactions with proteins like CD19 contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (ab243317), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (ab243317)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (ab243317), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [4B2] (ab243317)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

    Negative control: EL4 (PMID: 10453011).

  • Western blot - Anti-CD21+CD35 antibody [4B2] (ab243317), expandable thumbnail

    Western blot - Anti-CD21+CD35 antibody [4B2] (ab243317)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: EL4 (PMID: 10453011).

    All lanes: Western blot - Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/mL

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

    Lane 2: 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

    Predicted band size: 113 kDa

    Observed band size: 155 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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