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Rabbit Recombinant Monoclonal CD21 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (AB317494), expandable thumbnail
  • Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (AB317494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (AB317494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (AB317494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (AB317494), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow CytIP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.

Alternative names

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

Recommended products

Rabbit Recombinant Monoclonal CD21 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27417-508
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human WB

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The CD21+CD35 target commonly known as CD21/CD35 includes two surface proteins: CD21 and CD35. CD21 also known as complement receptor 2 (CR2) has an approximate mass of 145 kDa. CD35 also called complement receptor 1 (CR1) varies in mass between 190 to 280 kDa due to glycosylation. These proteins are expressed on the surface of B cells dendritic cells and some epithelial cells. Both markers participate in the immune system aiding in the clearance of pathogens and foreign antigens.

Biological function summary

CD21 and CD35 mediate the binding and clearance of opsonized antigens. These proteins form part of larger complexes where they act as receptors for complement proteins like C3d and C4b. CD21 enhances B cell activation by bridging the B-cell receptor with C3d-bound antigens. Meanwhile CD35 helps clear immune complexes reducing inflammation and facilitating healing.

Pathways

CD21/CD35 plays a significant role in the complement cascade and immune complex clearance pathways. Both CD21 and CD35 connect to other complement proteins such as C3. CD21 also interacts with proteins like CD19 and CD81 forming part of the B-cell co-receptor complex which amplifies B-cell receptor signaling and assists in immune response regulation.

Associated diseases and disorders

CD21/CD35 involvement links with autoimmune conditions such as systemic lupus erythematosus and chronic lymphocytic leukemia. In these diseases defective or altered CD21/CD35 expression can lead to inadequate immune complex clearance or abnormal B cell activation. Dysfunction in this system may alter interactions with proteins like CD19 contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494), expandable thumbnail

    Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)

    CD21+CD35 Western blot staining using rabbit Anti-CD21+CD35 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: EL4(PMID: 10453011)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 32907542)

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494) at 1/1000 dilution

    Lane 1: WEHI-231 (mouse B cell lymphoma B lymphocyte ) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 190 kDa, 250 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494), expandable thumbnail

    Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: thymus, EL4.IL-2 (PMID: 2966205)

    The identity of the lower MW band at approximately 37 kDa (in lane 1 and 2) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1-2: 180 seconds, Lane3-4: 15 seconds

    All lanes: Western blot - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494) at 1/1000 dilution

    Lane 1: A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse thymus tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 190 kDa, 250 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse liver.

    The section was incubated with ab317494 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human liver.

    The section was incubated with ab317494 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen.

    The section was incubated with ab317494 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [EPR27417-508] (ab317494)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD21+CD35 with ab317494 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human tonsil.

    The section was incubated with ab317494 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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