Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)

This data was developed using ab317022, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocyte cells labelling CD21+CD35 with ab317022 at 1/100 (4.99 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)

This data was developed using ab317022, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / WEHI-231 (mouse B cell lymphoma B lymphocyte, Right) cells labelling CD21+CD35 with ab317022 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cell.
Negative control : EL4(PMID : 10453011)

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)

This data was developed using ab317022, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of mouse splenocytes cells labelling CD21+CD35 with ab317022 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

mouse splenocytes are co-stained with CD3 conjugated Alexa Fluor®647.
Gated on viable cell.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)

This data was developed using ab317022, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of mouse splenocytes cells labelling CD21+CD35 with ab317022 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

mouse splenocytes are co-stained with CD19 conjugated Alexa Fluor®647.
Gated on viable cell.

• Dot

Supplier Data

Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)

This data was developed using ab317022, the same antibody clone in a different buffer formulation.

Dot blot analysis of CD21+CD35 using ab317022 at 1 : 1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] (<a href='/en-us/products/primary-antibodies/cd21cd35-antibody-epr27417-513-ab317022'>ab317022</a>) at 1/1000 dilution

All lanes:

Mouse CR2 protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s