Rabbit Recombinant Monoclonal CD21 antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt and reacts with Recombinant fragment - Mouse, Mouse samples.
Images
![Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--flow-cytometry-img429172.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)")
![Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--flow-cytometry-img429171.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)")
![Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--flow-cytometry-img429170.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--immunocytochemistry-immunofluorescence-img429152.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)")
![Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--dot-blot-img429110.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (AB317023)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | ICC/IF | Flow Cyt | WB | IP | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Target data
Function
Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.
Additional Targets
CR1
Alternative names
CD21, Complement receptor type 2, Cr2, Complement C3d receptor
Recommended products
Rabbit Recombinant Monoclonal CD21 antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt and reacts with Recombinant fragment - Mouse, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27417-513
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317023 is the carrirer-free version of Anti-CD21+CD35 antibody [EPR27417-513] ab317022.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--flow-cytometry-img429172.jpg "Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)")
Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)
This data was developed using Anti-CD21+CD35 antibody [EPR27417-513] ab317022, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / WEHI-231 (mouse B cell lymphoma B lymphocyte, Right) cells labelling CD21+CD35 with Anti-CD21+CD35 antibody [EPR27417-513] ab317022 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cell.
Negative control: EL4(PMID: 10453011)![Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--flow-cytometry-img429171.jpg "Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)")
Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)
This data was developed using Anti-CD21+CD35 antibody [EPR27417-513] ab317022, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of mouse splenocytes cells labelling CD21+CD35 with Anti-CD21+CD35 antibody [EPR27417-513] ab317022 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
mouse splenocytes are co-stained with CD19 conjugated Alexa Fluor®647.
Gated on viable cell.![Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--flow-cytometry-img429170.jpg "Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)")
Flow Cytometry - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)
This data was developed using Anti-CD21+CD35 antibody [EPR27417-513] ab317022, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of mouse splenocytes cells labelling CD21+CD35 with Anti-CD21+CD35 antibody [EPR27417-513] ab317022 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
mouse splenocytes are co-stained with CD3 conjugated Alexa Fluor®647.
Gated on viable cell.![Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--immunocytochemistry-immunofluorescence-img429152.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)")
Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)
This data was developed using Anti-CD21+CD35 antibody [EPR27417-513] ab317022, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocyte cells labelling CD21+CD35 with Anti-CD21+CD35 antibody [EPR27417-513] ab317022 at 1/100 (4.99 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023), expandable thumbnail](https://content.abcam.com/products/images/cd21cd35-antibody-epr27417-513-bsa-and-azide-free-ab317023--dot-blot-img429110.jpg "Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)")
Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free (ab317023)
This data was developed using Anti-CD21+CD35 antibody [EPR27417-513] ab317022, the same antibody clone in a different buffer formulation.
Dot blot analysis of CD21+CD35 using Anti-CD21+CD35 antibody [EPR27417-513] ab317022 at 1:1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
All lanes: Dot Blot - Anti-CD21+CD35 antibody [EPR27417-513] (Anti-CD21+CD35 antibody [EPR27417-513] ab317022) at 1/1000 dilution
All lanes: Mouse CR2 protein with 5% NFDM/TBST
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com