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Rabbit Recombinant Multiclonal CD21 antibody. Suitable for ICC/IF, WB, IP, Flow Cyt, IHC-P, mIHC and reacts with Mouse samples.

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Images

Multiplex immunohistochemistry - Anti-CD21+CD35 antibody [RM2062] (AB318999), expandable thumbnail
  • Flow Cytometry - Anti-CD21+CD35 antibody [RM2062] (AB318999), expandable thumbnail
  • Flow Cytometry - Anti-CD21+CD35 antibody [RM2062] (AB318999), expandable thumbnail
  • Immunoprecipitation - Anti-CD21+CD35 antibody [RM2062] (AB318999), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [RM2062] (AB318999), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIPFlow CytIHC-PmIHC
Mouse
Tested
Tested
Tested
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.

Additional Targets

CR1

Alternative names

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

Recommended products

Rabbit Recombinant Multiclonal CD21 antibody. Suitable for ICC/IF, WB, IP, Flow Cyt, IHC-P, mIHC and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM2062
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes human CD21 in IHC-P and WB.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Multiplex immunohistochemistry - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    CD21+CD35 Multiplex immunohistochemistry staining of Mouse spleen using rabbit Anti-CD21+CD35 antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining CD21+CD35 with ab318999 at a 1:2000 (0.255 ug/ml) dilution, Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1:1000 (0.444 ug/ml) dilution and Anti-CD3 epsilon antibody [SP7] ab16669 anti-CD3 used at a 1:150 (0.06 ug/ml) dilution.

    Panel A: merged staining of anti-CD21+CD35 (green; Opal™520), anti-CD19 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on mouse spleen.

    Panel B: anti-CD21+CD35 staining B lymphocytes in mouse spleen.

    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.

    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab318999, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Flow Cytometry - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    Flow cytometric analysis of Mouse spleen cells labelling CD21+CD35 with ab318999 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse spleen cell are co-stained with CD19 conjugated PE/Cy7 (780/60BP).

    Gated on viable cell.

  • Flow Cytometry - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Flow Cytometry - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    Flow cytometric analysis of Mouse spleen cells labelling CD21+CD35 with ab318999 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse spleen cell are co-stained with CD3 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Immunoprecipitation - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Immunoprecipitation - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    CD21+CD35 was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab318999 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318999 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate 10ug

    Lane 2: ab318999 IP in Mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab318999 in mouse spleen tissue lysate

    All lanes: Immunoprecipitation - Anti-CD21+CD35 antibody [RM2062] (ab318999) at 1/30 dilution

    All lanes: Mouse spleen tissue lysate 10ug with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling CD21+CD35 with ab318999 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing membranous staining in mouse splenocytes (shown in green). The counterstain was CD45R/B220 which is observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-human/mouse CD45R/B220 rat monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD21+CD35 with ab318999 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen.

    The section was incubated with ab318999 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Western blot - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    Negative control: EL4(PMID: 10453011)

    Low expression: thymus, EL4.IL-2 (PMID: 2966205)

    The identity of the lower MW band at approximately 37 kDa (in lane1- 4) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1-4: 180 seconds, Lane5-6: 26 seconds

    All lanes: Western blot - Anti-CD21+CD35 antibody [RM2062] (ab318999) at 1/1000 dilution

    Lane 1: WEHI-231 (mouse B cell lymphoma B lymphocyte ) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: A20 (mouse reticum sarcoma b lymphocyte) whole cell lysate at 20 µg with NFDM/TBST

    Lane 4: EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with NFDM/TBST

    Lane 5: Mouse spleen tissue lysate at 20 µg with NFDM/TBST

    Lane 6: Mouse thymus tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 190 kDa, 250 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [RM2062] (ab318999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21+CD35 antibody [RM2062] (ab318999)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD21+CD35 with ab318999 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse liver.

    The section was incubated with ab318999 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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