Mouse Monoclonal CD22 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD22 aa 50-200.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109541.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755)")
![Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--western-blot-img158311.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755)")
![Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--western-blot-img17221.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21358.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755)")
![Protein Array - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--protein-array-img141174.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (AB269755)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD22 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P20273
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
7 products for Alternative Product
Target data
Function
Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:34330755, PubMed:8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed:20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed:8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed:20516366).
Alternative names
CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2
Recommended products
Mouse Monoclonal CD22 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD22 aa 50-200.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD22 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P20273
- Carrier free
- Yes
- Clone number
- BLCAM/1795
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab269755 is a carrier free version of Anti-CD22 antibody [BLCAM/1795] ab218340.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
- Biological function summary
CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.
- Pathways
CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.
- Associated diseases and disorders
CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109541.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD22 with Anti-CD22 antibody [BLCAM/1795] ab218340.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD22 antibody [BLCAM/1795] ab218340).
![Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--western-blot-img158311.jpg "Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)")
Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)
All lanes: Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755) at 2 µg/mL
Lane 1: Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2: Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Predicted band size: 95 kDa
![Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--western-blot-img17221.jpg "Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)")
Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)
All lanes: Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755) at 2 µg/mL
All lanes: Raji (human Burkitt's lymphoma cell line) whole cell lysate
Predicted band size: 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21358.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD22 with Anti-CD22 antibody [BLCAM/1795] ab218340 at 2 μg/ml dilution, followed by conjugation to the secondary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD22 antibody [BLCAM/1795] ab218340).
![Protein Array - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-blcam-1795-bsa-and-azide-free-ab269755--protein-array-img141174.jpg "Protein Array - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)")
Protein Array - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)
Anti-CD22 antibody [BLCAM/1795] ab218340 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD22 antibody [BLCAM/1795] ab218340).
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