AB207727

Anti-CD22 antibody [EPR20061]

Name: Anti-CD22 antibody [EPR20061] (ab207727) | Abcam
Brand: Abcam Limited
SKU: AB207727
Price: 215 USD
Availability: InStock
Rating: 4 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal CD22 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

10 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] (AB207727)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] (AB207727)

Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling CD22 with ab207727 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Daudi cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] (AB207727)

Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling CD22 with ab207727 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Raji cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling CD22 with ab207727 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : no staining on human thymoma [PMID : 11967115].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD22 with ab207727 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous and cytoplasmic staining on B cells of human spleen [PMID : 11967115]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD22 with ab207727 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous and cytoplasmic staining on B cells of human tonsil [PMID : 11967115]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-CD22 antibody [EPR20061] (AB207727)

CD22 was immunoprecipitated from 0.35 mg of Human tonsil lysate with ab207727 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207727 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil lysate, 10 μg (Input).

Lane 2 : ab207727 IP in Human tonsil lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207727 in Human tonsil lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD22 antibody [EPR20061] (ab207727)

Predicted band size: 95 kDa

Observed band size: 130 kDa

false

Supplier Data

Immunoprecipitation - Anti-CD22 antibody [EPR20061] (AB207727)

CD22 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab207727 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207727 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Raji whole cell lysate, 10 μg (Input).

Lane 2 : ab207727 IP in Raji whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207727 in Raji whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD22 antibody [EPR20061] (ab207727)

Predicted band size: 95 kDa

Observed band size: 130 kDa

false

Supplier Data

Western blot - Anti-CD22 antibody [EPR20061] (AB207727)

All lanes:

Western blot - Anti-CD22 (phospho Y807) antibody [Y505] (<a href='/en-us/products/primary-antibodies/cd22-phospho-y807-antibody-y505-ab32040'>ab32040</a>) at 10000 kDa

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) treated with 10mM Pervanadate for 30 min whole cell lysate at 15 µg

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) treated with 10mM Pervanadate for 30 min whole cell lysate, then the membrane was incubated with alkaline phosphatase for 1 hour at 15 µg

Predicted band size: 95 kDa

Observed band size: 150 kDa

false

Exposure time: 40s

Supplier Data

Western blot - Anti-CD22 antibody [EPR20061] (AB207727)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 seconds; Lane 2/3 : 1 second; Lane 4 : 8 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24264377 & 21178016).

Lanes 1 and 4:

Western blot - Anti-CD22 antibody [EPR20061] (ab207727) at 1/2000 dilution

Lanes 2 - 3:

Western blot - Anti-CD22 antibody [EPR20061] (ab207727) at 1/10000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 3:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 4:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 95 kDa

Observed band size: 130 kDa

false

Supplier Data

Western blot - Anti-CD22 antibody [EPR20061] (AB207727)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24264377 & 21178016).

All lanes:

Western blot - Anti-CD22 antibody [EPR20061] (ab207727) at 1/1000 dilution

All lanes:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 95 kDa

Observed band size: 130 kDa

false

Exposure time: 3min

Carrier free

Anti-CD22 antibody [EPR20061] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20061

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).

See full target information CD22

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3516 PubMed40229254

2025

Parallel comparison of T cell and B cell subpopulations of adenoid hypertrophy and tonsil hypertrophy of children.

Applications

Unspecified application

Species

Unspecified reactive species

Zihui Yu,Ziying Xu,Tongtong Fu,Shiyu Liu,Jinghua Cui,Bing Zhang,Jieqiong Liang,Chong Pang,Yuehua Ke,Ruikun Wang,Zhijie Tang,Yagang Gao,Bing Du,Yanling Feng,Hanqing Zhao,Guanhua Xue,Chao Yan,Lin Gan,Junxia Feng,Zheng Fan,Yang Yang,Lijuan Huang,Shuo Zhao,Sun Ying,Qinglong Gu,Jing Yuan

Journal of neuroinflammation 22:32 PubMed39910617

2025

CD22 modulation alleviates amyloid β-induced neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Dong Mai,Qingqing Zhang,Cheuk Lim Fung,Shui On Leung,Chi Ho Chong

Cell reports. Medicine 5:101627 PubMed38964315

2024

Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Shangru Li,Lefan Zhu,Mingle Huang,Yubin Xie,Xinming Song,Zhihui Chen,Harry Cheuk-Hay Lau,Joseph Jao-Yiu Sung,Lixia Xu,Jun Yu,Xiaoxing Li

Journal of translational medicine 21:710 PubMed37817249

2023

CD22 is a potential target of CAR-NK cell therapy for esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingdang Liu,Ximing Dai,Yien Xu,Tian Guan,Liangli Hong,Tahir Zaib,Qi Zhou,Ke Cheng,Xiaoling Zhou,Changchun Ma,Pingnan Sun

Glycobiology 33:732-744 PubMed37498177

2023

A photoaffinity glycan-labeling approach to investigate immunoglobulin glycan-binding partners.

Applications

Unspecified application

Species

Unspecified reactive species

Miles D Holborough-Kerkvliet,Greta Mucignato,Sam J Moons,Venetia Psomiadou,Rohit S R Konada,Nichole J Pedowitz,Matthew R Pratt,Theresa Kissel,Carolien A M Koeleman,Rayman T N Tjokrodirijo,Petrus A van Veelen,Thomas Huizinga,Karin A J van Schie,Manfred Wuhrer,Jennifer J Kohler,Kimberly M Bonger,Thomas J Boltje,Reinaldus E M Toes

International journal of molecular sciences 24: PubMed36768478

2023

Expression of CD22 in Triple-Negative Breast Cancer: A Novel Prognostic Biomarker and Potential Target for CAR Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tahir Zaib,Ke Cheng,Tingdang Liu,Ruyi Mei,Qin Liu,Xiaoling Zhou,Lifang He,Hibba Rashid,Qingdong Xie,Hanif Khan,Yien Xu,Pingnan Sun,Jundong Wu

NPJ Regenerative medicine 7:14 PubMed35110584

2022

CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Junghof,Yuta Kogure,Tian Yu,Eva María Verdugo-Sivianes,Megumi Narita,Antonio Lucena-Cacace,Yoshinori Yoshida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD22 antibody [EPR20061]

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] (AB207727)

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR20061] (AB207727)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [EPR20061] (AB207727)

Immunoprecipitation - Anti-CD22 antibody [EPR20061] (AB207727)

Immunoprecipitation - Anti-CD22 antibody [EPR20061] (AB207727)

Western blot - Anti-CD22 antibody [EPR20061] (AB207727)

Western blot - Anti-CD22 antibody [EPR20061] (AB207727)

Western blot - Anti-CD22 antibody [EPR20061] (AB207727)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

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Biological function summary

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Product protocols

Target data

Publications (7)

Product promise