Anti-CD22 antibody [EPR30959-587]

• Flow Cyt

Lab

Flow Cytometry - Anti-CD22 antibody [EPR30959-587] (AB326045)

Flow cytometric analysis of Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling CD22 with ab326045 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®647.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD22 antibody [EPR30959-587] (AB326045)

Flow cytometric analysis of Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling CD22 with ab326045 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD19 conjugated to PE/CY7.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD22 antibody [EPR30959-587] (AB326045)

Flow cytometric analysis of WEHI-231 (mouse B cell lymphoma B lymphocyte, Right) and J558 (mouse B lymphocyte lymphoblast, Left) cells labelling with ab326045 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : J558.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR30959-587] (AB326045)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling with ab326045 at 1/1000 (0.514 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subsets of CD3 negative mouse PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-rat CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain CD3 at 1/100 dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [EPR30959-587] (AB326045)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231(mouse B cell lymphoma B lymphocyte) and J558 (mouse B lymphocyte lymphoblast) cells labelling with ab326045 at 1/1000 (0.514 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in WEHI-231 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Low expression : J558 (PMID : 11807774). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab326045 at 1/1000 dilution, followed by ab150120 at 1/1000 dilution.

-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 at 1/1000 dilution.

• I-ELISA

Lab

Indirect ELISA - Anti-CD22 antibody [EPR30959-587] (AB326045)

Indirect ELISA analysis of ab326045 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific at 1 : 20000 dilution.

Antigen : Mouse B-cell receptor CD22 recombinant protein.

Antigen concentration : 1000 ng/ml