Rat Recombinant Monoclonal CD22 antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.
Images
![Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (AB244594), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-ox97-bsa-and-azide-free-ab244594--flow-cytometry-img37593.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (AB244594)")
![Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (AB244594), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-ox97-bsa-and-azide-free-ab244594--immunohistochemistry-frozen-sections-img119858.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (AB244594)")
Publications
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- European journal of immunology 32:1924-322002Comparison of CD22 binding to native CD45 and synthetic oligosaccharide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTalitha R Bakker,Christina Piperi,Elizabeth A Davies,P Anton van der MerwePubMed 12115612
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-Fr | |
|---|---|---|
| Mouse | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.2 µg/mL | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:7533044, PubMed:9016707). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration. Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation (PubMed:9016707). Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling. If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced.
Alternative names
CD22, Lyb-8, Siglec2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2
Recommended products
Rat Recombinant Monoclonal CD22 antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- OX97
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
ab244594 is the carrier free version of Anti-CD22 antibody [OX97] ab243840.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
- Biological function summary
CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.
- Pathways
CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.
- Associated diseases and disorders
CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-ox97-bsa-and-azide-free-ab244594--flow-cytometry-img37593.jpg "Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)")
Flow Cytometry - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD22 antibody [OX97] ab243840).
C57BL/6 mouse splenocytes stained with Anti-CD22 antibody [OX97] ab243840 (right) or Rat IgG1κ (ab18407) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-CD22 antibody [OX97] ab243840) or Rat IgG1κ (ab18407) (1x106 in 100μl at 0.2 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165) was used at 1/2000 dilution for 30 min at 4°C.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
![Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594), expandable thumbnail](https://content.abcam.com/products/images/cd22-antibody-ox97-bsa-and-azide-free-ab244594--immunohistochemistry-frozen-sections-img119858.jpg "Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)")
Immunohistochemistry (Frozen sections) - Anti-CD22 antibody [OX97] - BSA and Azide free (ab244594)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD22 antibody [OX97] ab243840).
IHC image of CD22 staining in a section of frozen normal mouse spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Anti-CD22 antibody [OX97] ab243840 at 1μg/ml and Anti-CD4 antibody [EPR19514] ab183685 (Rabbit monoclonal [EPR19514] to CD4) at 1/200, to show the distinct staining of B cells and T cells. The section was then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 (Goat Anti-Rabbit IgG H&L (Alexa Fluor®594) (Shown in red) 1/1000) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. DAPI was used to stain the cell nuclei (blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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