AB227645

Anti-CD22 antibody [SP104] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD22 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD22 using ab227645 at 1/100 dilution in immunohistochemical analysis.

Carrier free

Anti-CD22 antibody [SP104] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD22 antibody [SP104] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD22 antibody [SP104] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD22 antibody [SP104] - C-terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD22 antibody [SP104] - C-terminal
578 PE

PE Anti-CD22 antibody [SP104] - C-terminal
660 APC

APC Anti-CD22 antibody [SP104] - C-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD22 antibody [SP104] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP104

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).

See full target information CD22

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 82:648-664 PubMed34853070

2021

Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge.

Applications

Unspecified application

Species

Unspecified reactive species

Gina Bouchard,Fernando Jose Garcia-Marques,Loukia Georgiou Karacosta,Weiruo Zhang,Abel Bermudez,Nicholas McIlvain Riley,Sushama Varma,Lindsey Catherine Mehl,Jalen Anthony Benson,Joseph B Shrager,Carolyn Ruth Bertozzi,Sharon J Pitteri,Amato J Giaccia,Sylvia Katina Plevritis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD22 antibody [SP104] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [SP104] - C-terminal (AB227645)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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