AB32123

Anti-CD22 (phospho Y822) antibody [Y506]

Name: Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) | Abcam
Brand: Abcam Limited
SKU: AB32123
Price: 510 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
What is this?

(1 Review)

(3 Publications)

Rabbit Monoclonal CD22 phospho Y822 antibody. Suitable for Flow Cyt, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 3 publications.

View Alternative Names

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

4 Images

Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using ab32123

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Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

All lanes:

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

Lane 1:

Untreated Raji cell lysate

Lane 2:

Raji cell lysate treated with Pervanadate

Predicted band size: 95 kDa

Observed band size: 150 kDa

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Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

Lane 2:

Ramos treated with 1mM pervanadate for 30 min whole cell lysate at 10 µg

Lane 3:

Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 150 kDa

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Unknown

Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Dot blot analysis of CD22 (phospho Y822) phospho peptide (Lane 1) and CD22 non-phospho peptide (Lane 2) labelling CD22 (phospho Y822) with ab32123 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y506

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).

See full target information CD22 phospho Y822

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 24:1762-1777 PubMed37653247

2023

Molecular basis for potent B cell responses to antigen displayed on particles of viral size.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy F Brooks,Julianne Riggs,James L Mueller,Raisa Mathenge,Wei-Yun Wholey,Alexander R Meyer,Sekou-Tidiane Yoda,Vivasvan S Vykunta,Hailyn V Nielsen,Wei Cheng,Julie Zikherman

Proceedings of the National Academy of Sciences of 112:E6515-24 PubMed26561584

2015

An extracatalytic function of CD45 in B cells is mediated by CD22.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Coughlin,Mark Noviski,James L Mueller,Ammarina Chuwonpad,William C Raschke,Arthur Weiss,Julie Zikherman

Blood 116:1698-704 PubMed20516366

2010

IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes.

Applications

Flow Cyt

Species

Human

Jean-François Séïté,Divi Cornec,Yves Renaudineau,Pierre Youinou,Rizgar A Mageed,Sophie Hillion

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD22 (phospho Y822) antibody [Y506]

Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise