Rabbit Monoclonal CD22 phospho Y822 antibody. Carrier free. Suitable for Flow Cyt, Dot, WB and reacts with Human, Synthetic peptide samples.
Images
![Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--flow-cytometry-img73802.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667)")
![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--western-blot-img39212.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667)")
![Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--dot-blot-img21673.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667)")
![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--western-blot-img65384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (AB284667)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC | Flow Cyt | Dot | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Tested |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:34330755, PubMed:8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed:20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed:8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed:20516366).
Alternative names
CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2
Recommended products
Rabbit Monoclonal CD22 phospho Y822 antibody. Carrier free. Suitable for Flow Cyt, Dot, WB and reacts with Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- Y506
- Purification technique
- Affinity purification Protein A
- Specificity
ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284667 is the carrier-free version of Anti-CD22 (phospho Y822) antibody [Y506] ab32123
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
- Biological function summary
CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.
- Pathways
CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.
- Associated diseases and disorders
CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--flow-cytometry-img73802.jpg "Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)")
Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)
This data was developed using Anti-CD22 (phospho Y822) antibody [Y506] ab32123, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using Anti-CD22 (phospho Y822) antibody [Y506] ab32123
![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--western-blot-img39212.jpg "Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)")
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)
This data was developed using Anti-CD22 (phospho Y822) antibody [Y506] ab32123, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (Anti-CD22 (phospho Y822) antibody [Y506] ab32123) at 1/1000 dilution
Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg
Lane 2: Ramos treated with 1mM pervanadate for 30 min whole cell lysate at 10 µg
Lane 3: Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 150 kDa
![Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--dot-blot-img21673.jpg "Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)")
Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)
This data was developed using Anti-CD22 (phospho Y822) antibody [Y506] ab32123, the same antibody clone in a different buffer formulation.Dot blot analysis of CD22 (phospho Y822) phospho peptide (Lane 1) and CD22 non-phospho peptide (Lane 2) labelling CD22 (phospho Y822) with Anti-CD22 (phospho Y822) antibody [Y506] ab32123 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667), expandable thumbnail](https://content.abcam.com/products/images/cd22-phospho-y822-antibody-y506-bsa-and-azide-free-ab284667--western-blot-img65384.jpg "Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)")
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free (ab284667)
This data was developed using Anti-CD22 (phospho Y822) antibody [Y506] ab32123, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (Anti-CD22 (phospho Y822) antibody [Y506] ab32123) at 1/1000 dilution
Lane 1: Untreated Raji cell lysate
Lane 2: Raji cell lysate treated with Pervanadate
Predicted band size: 95 kDa
Observed band size: 150 kDa
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