Rabbit Recombinant Monoclonal CD226 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD226 aa 1-250.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline
Liquid
Monoclonal
ICC | IP | WB | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Use 6 μl/mg lysate. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
DNAM1, CD226, CD226 antigen, DNAX accessory molecule 1, DNAM-1
Rabbit Recombinant Monoclonal CD226 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD226 aa 1-250.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline
Liquid
Monoclonal
BLR063G
Purified from cell culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is sold under License from Bethyl Laboratories, Inc.
This supplementary information is collated from multiple sources and compiled automatically.
CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.
CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.
CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Formalin-fixed, paraffin-embedded Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for CDD226 using ab264415 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Formalin-fixed, paraffin embedded human lung carcinoma tissue stained for CD226 using ab264415 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
CD226 was immunoprecipitated from HH whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264415 at 6 μl per reaction. Western blot was performed on the immunoprecipitates using ab264415 at 1/1000 dilution.
Lane 1: ab264415 IP in HH cell lysate.
Lane 2: Control IgG IP in HH cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 3s
Formalin-fixed, paraffin-embedded HH cells stained for CDD226 using ab264415 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-CD226 antibody [BLR063G] - BSA free (ab264415) at 1/1000 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) whole cell lysate at 50 µg
Lane 2: SUP-M2 whole cell lysate at 50 µg
Lane 3: U-251 MG (human brain glioma cell line) whole cell lysate at 50 µg
Lane 4: HH whole cell lysate at 50 µg
Lane 5: MJ whole cell lysate at 50 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 7: HDLM-2 whole cell lysate at 50 µg
Lane 8: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 3s
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