AB264415

Anti-CD226 antibody [BLR063G] - BSA free

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD226 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD226 aa 1-250.

View Alternative Names

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1

5 Images

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Immunocytochemistry - Anti-CD226 antibody [BLR063G] - BSA free (AB264415)

Formalin-fixed, paraffin-embedded HH cells stained for CDD226 using ab264415 at 1/200 dilution in imunocytochemical analysis. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [BLR063G] - BSA free (AB264415)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [BLR063G] - BSA free (AB264415)

Formalin-fixed, paraffin embedded human lung carcinoma tissue stained for CD226 using ab264415 at 1/200 dilution in immunohistochemical analysis. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

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Immunocytochemistry - Anti-CD226 antibody [BLR063G] - BSA free (AB264415)

Formalin-fixed, paraffin-embedded Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for CDD226 using ab264415 at 1/200 dilution in imunocytochemical analysis. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-CD226 antibody [BLR063G] - BSA free (AB264415)

CD226 was immunoprecipitated from HH whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264415 at 6 μl per reaction. Western blot was performed on the immunoprecipitates using ab264415 at 1/1000 dilution.

Lane 1 : ab264415 IP in HH cell lysate.

Lane 2 : Control IgG IP in HH cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

Predicted band size: 39 kDa

true

Exposure time: 3s

Supplier Data

Western blot - Anti-CD226 antibody [BLR063G] - BSA free (AB264415)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CD226 antibody [BLR063G] - BSA free (ab264415) at 1/1000 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate at 50 µg

Lane 2:

SUP-M2 whole cell lysate at 50 µg

Lane 3:

U-251 MG (human brain glioma cell line) whole cell lysate at 50 µg

Lane 4:

HH whole cell lysate at 50 µg

Lane 5:

MJ whole cell lysate at 50 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 7:

HDLM-2 whole cell lysate at 50 µg

Lane 8:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 39 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR063G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CD226 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q15762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 6 μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections." } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.

Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

See full target information CD226

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 17:3320-3330 PubMed34512149

2021

NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis inhibiting the CTHRC1/HIF-1α axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Yang Yang,Haiying Yan,Xiaying Peng,Jun Zou

View all publications

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Product promise

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