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AB33397

Anti-CD226 antibody [DX11]

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(8 Publications)

Mouse Monoclonal CD226 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing CD226 protein.

View Alternative Names

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1

1 Images
Flow Cytometry - Anti-CD226 antibody [DX11] (AB33397)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD226 antibody [DX11] (AB33397)

Human peripheral blood lymphocytes stained with ab33397, using 10µl of the working dilution for 106 cells in 100µl.

  • 519 FITC

    FITC Anti-CD226 antibody [DX11]

  • 578 PE

    PE Anti-CD226 antibody [DX11]

  • Biotin

    Biotin Anti-CD226 antibody [DX11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DX11

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD226 protein. The exact immunogen used to generate this antibody is proprietary information.

Q15762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
See full target information CD226
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 12:1421-1437 PubMed38949179

2024

NK Receptor Signaling Lowers TCR Activation Threshold, Enhancing Selective Recognition of Cancer Cells by TAA-Specific CTLs.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Dong,Nataša Obermajer,Takemasa Tsuji,Junko Matsuzaki,Cindy M Bonura,Cindy Sander,Henry Withers,Mark D Long,Colin Chavel,Scott H Olejniczak,Hans Minderman,John M Kirkwood,Robert P Edwards,Walter J Storkus,Pedro Romero,Pawel Kalinski

Cancer immunology, immunotherapy : CII 66:1367-1375 PubMed28623459

2017

Blockade of CD112R and TIGIT signaling sensitizes human natural killer cell functions.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Xu,Alexander Sunderland,Yue Zhou,Richard D Schulick,Barish H Edil,Yuwen Zhu

International journal of oncology 48:1794-804 PubMed26936487

2016

Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Sugai,Toshiaki Yoshikawa,Tatsuaki Iwama,Nobuhiro Tsuchiya,Norihiro Ueda,Norihiro Fujinami,Manami Shimomura,Rong Zhang,Shin Kaneko,Yasushi Uemura,Tetsuya Nakatsura

The Journal of experimental medicine 213:167-76 PubMed26755705

2016

Identification of CD112R as a novel checkpoint for human T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwen Zhu,Alessandro Paniccia,Alexander C Schulick,Wei Chen,Michelle R Koenig,Joshua T Byers,Sheng Yao,Shaun Bevers,Barish H Edil

Journal of immunology (Baltimore, Md. : 1950) 191:3673-80 PubMed23980210

2013

The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans.

Applications

BL

Species

Human

Ester Lozano,Nicole Joller,Yonghao Cao,Vijay K Kuchroo,David A Hafler

Cancer research 73:4653-62 PubMed23761327

2013

IL-18-primed helper NK cells collaborate with dendritic cells to promote recruitment of effector CD8+ T cells to the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey L Wong,Erik Berk,Robert P Edwards,Pawel Kalinski

Journal of immunology (Baltimore, Md. : 1950) 188:3869-75 PubMed22427644

2012

The TIGIT/CD226 axis regulates human T cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Lozano,Margarita Dominguez-Villar,Vijay Kuchroo,David A Hafler

Analytical biochemistry 386:237-43 PubMed19135971

2009

Use of tandem Biacore-mass spectrometry to identify platelet membrane targets of novel monoclonal antibodies.

Applications

Flow Cyt

Species

Human

Catherine Ravanat,Virginie Wurtz,Philippe Ohlmann,Marie Fichter,Jean-Pierre Cazenave,Alain Vandorsselaer,François Lanza,Christian Gachet
View all publications
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