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Rabbit Recombinant Monoclonal CD226 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR20710] (AB212077), expandable thumbnail
  • Western blot - Anti-CD226 antibody [EPR20710] (AB212077), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (AB212077), expandable thumbnail
  • Immunoprecipitation - Anti-CD226 antibody [EPR20710] (AB212077), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (AB212077), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Mouse
Tested
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding.

Alternative names

CD226 antigen, Platelet and T-cell activation antigen 1, Pta1, Cd226

Recommended products

Rabbit Recombinant Monoclonal CD226 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20710

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.

Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

Associated diseases and disorders

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR20710] (ab212077), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR20710] (ab212077)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte cell line) cells labeling CD226 with ab212077 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)(Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on EL4.IL-2 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-CD226 antibody [EPR20710] (ab212077), expandable thumbnail

    Western blot - Anti-CD226 antibody [EPR20710] (ab212077)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 1 minute; Lane 3: 3 minutes.

    The different molecular weights (~65kDa) observed in different tissues or cell lines may be due to different level of CD226 glycosylation (PMID: 8673704).

    All lanes: Western blot - Anti-CD226 antibody [EPR20710] (ab212077) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: EL4.IL-2 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg

    Lane 3: YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 65 kDa

    This data was developed using ab212077, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 1 minute; Lane 3: 3 minutes.

    The different molecular weights (~65kDa) observed in different tissues or cell lines may be due to different level of CD226 glycosylation (PMID: 8673704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (ab212077), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (ab212077)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD226 with ab212077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed (PMID: 15693793). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CD226 antibody [EPR20710] (ab212077), expandable thumbnail

    Immunoprecipitation - Anti-CD226 antibody [EPR20710] (ab212077)

    CD226 was immunoprecipitated from 0.35 mg of mouse spleen lysate with ab212077 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212077 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse spleen lysate 10 μg (Input).

    Lane 2: ab212077 IP in mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab212077 in mouse spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    All lanes: Immunoprecipitation - Anti-CD226 antibody [EPR20710] (ab212077)

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (ab212077), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (ab212077)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD226 with ab212077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Positive staining on platelets of mouse lung is observed (PMID: 15693793). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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