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Rabbit Recombinant Monoclonal CD226 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR20710] - BSA and Azide free (AB251537), expandable thumbnail
  • Western blot - Anti-CD226 antibody [EPR20710] - BSA and Azide free (AB251537), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (AB251537), expandable thumbnail
  • Immunoprecipitation - Anti-CD226 antibody [EPR20710] - BSA and Azide free (AB251537), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (AB251537), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Mouse
Tested
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:19029379, PubMed:24658051, PubMed:26552706). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

Alternative names

CD226, Pta1, Cd226, CD226 antigen, Platelet and T-cell activation antigen 1

Recommended products

Rabbit Recombinant Monoclonal CD226 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20710
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251537 is the carrier-free version of Anti-CD226 antibody [EPR20710] ab212077.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.

Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

Associated diseases and disorders

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

    This data was developed using Anti-CD226 antibody [EPR20710] ab212077, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte cell line) cells labeling CD226 with Anti-CD226 antibody [EPR20710] ab212077 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)(Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on EL4.IL-2 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537), expandable thumbnail

    Western blot - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

    This data was developed using Anti-CD226 antibody [EPR20710] ab212077, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 1 minute; Lane 3: 3 minutes.

    The different molecular weights (~65kDa) observed in different tissues or cell lines may be due to different level of CD226 glycosylation (PMID: 8673704).

    All lanes: Western blot - Anti-CD226 antibody [EPR20710] (Anti-CD226 antibody [EPR20710] ab212077) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: EL4.IL-2 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg

    Lane 3: YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

    This data was developed using Anti-CD226 antibody [EPR20710] ab212077, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD226 with Anti-CD226 antibody [EPR20710] ab212077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed (PMID: 15693793). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537), expandable thumbnail

    Immunoprecipitation - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

    This data was developed using Anti-CD226 antibody [EPR20710] ab212077, the same antibody clone in a different buffer formulation.

    CD226 was immunoprecipitated from 0.35 mg of mouse spleen lysate with Anti-CD226 antibody [EPR20710] ab212077 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CD226 antibody [EPR20710] ab212077 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse spleen lysate 10 μg (Input).

    Lane 2: Anti-CD226 antibody [EPR20710] ab212077 IP in mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD226 antibody [EPR20710] ab212077 in mouse spleen lysate

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    All lanes: Immunoprecipitation - Anti-CD226 antibody [EPR20710] (Anti-CD226 antibody [EPR20710] ab212077)

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] - BSA and Azide free (ab251537)

    This data was developed using Anti-CD226 antibody [EPR20710] ab212077, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD226 with Anti-CD226 antibody [EPR20710] ab212077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Positive staining on platelets of mouse lung is observed (PMID: 15693793). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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