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AB89394

Anti-CD226 antibody [MM0248- 1X20]

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(1 Publication)

Mouse Monoclonal CD226 antibody. Suitable for WB, FuncS (Neut/Block) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD226.

View Alternative Names

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0248- 1X20

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, FuncS (Neut/Block)

applications

Immunogen

Recombinant Fragment Protein within Human CD226. The exact immunogen used to generate this antibody is proprietary information.

Q15762

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Alternative Products

Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB230865

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Primary Antibodies

AB33397

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Complementary Products

Assay Kits

AB282303

Human CD226 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD226 ELISA Kit (AB282303)

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Primary Antibodies

AB267788

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151]

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Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB52587

Anti-PD1 antibody [NAT105]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
See full target information CD226
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0139603 PubMed26441059

2015

Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Wolpert,Caroline Happold,Guido Reifenberger,Ana-Maria Florea,René Deenen,Patrick Roth,Marian Christoph Neidert,Katrin Lamszus,Manfred Westphal,Michael Weller,Günter Eisele
View all publications
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Product promise

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