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Rabbit Recombinant Monoclonal CD23 antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications.

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Images

Flow Cytometry - Anti-CD23 antibody [EPR3617] (AB92495), expandable thumbnail
  • Western blot - Anti-CD23 antibody [EPR3617] (AB92495), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (AB92495), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (AB92495), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (AB92495), expandable thumbnail

Publications

  • Frontiers in immunology 14:11428622023
    Immune desert in MMR-deficient tumors predicts poor responsiveness of immune checkpoint inhibition.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Guoxing Zheng,Yingsi Lu,Zheng Yang,Hong Chen,Qian Liang,Qingqing Zhu,Yan Li,Xing Xiao,Zhuzhen He,Yifan Zhu,Bo Li,Leilei Huang,Nan Dong,Shuang Hu,Yihang Pan,Changhua Zhang,Chengming Zhu
    PubMed 37187745
  • Frontiers in oncology 10:6098392021
    Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Shuang Wen,Tianqing Liu,Hongshuo Zhang,Xu Zhou,Huidan Jin,Man Sun,Zhifei Yun,Hong Luo,Ze Ni,Rui Zhao,Bo Fan
    PubMed 33585230

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/400

Notes

For unpurified use at 1/00 - 1/250.

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/20

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

      Alternative names

      Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, FCER2, IGEBF, FCE2, CD23A, CLEC4J

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      Rabbit Recombinant Monoclonal CD23 antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications.

      Alternative names

      Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, FCER2, IGEBF, FCE2, CD23A, CLEC4J

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR3617

      Purification technique

      Affinity purification Protein A

      Specificity

      Recognizes both membrane bound and soluble forms of CD23.

      Dissociation constant

      5.5 x 10-12 M

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.

      Biological function summary

      CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

      Pathways

      CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

      Associated diseases and disorders

      CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      9 product images

      • Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)

        Flow Cytometry analysis of Human peripheral blood mononuclear (PBMC) cells labeling CD23 with purified ab92495 at 1:20 dilution (10 μg/ml) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left).

        Cells were stained with rabbit IgG (Left) or ab92495 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

      • Western blot - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Western blot - Anti-CD23 antibody [EPR3617] (ab92495)

        The bands detected by ab92495 are consistent with what have been described in PMID: 20876574 and PMID: 17389606.

        All lanes: Western blot - Anti-CD23 antibody [EPR3617] (ab92495) at 1/1000 dilution

        All lanes: Human lymph lysates at 15 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 36 kDa

        Observed band size: 37 kDa, 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD23 with Purified ab92495 at 1/400 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

        ab92495 (unpurified), at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.

        Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

        ab92495 (unpurified) showing positive staining in Normal tonsil tissue.

        Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

        ab92495 (unpurified) showing negative staining in Normal heart tissue.


        Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

        ab92495 (unpurified) showing negative staining in Normal breast tissue.


        Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

        ab92495 (unpurified) showing negative staining in Normal brain tissue.


        Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-CD23 antibody [EPR3617] (ab92495), expandable thumbnail

        OI-RD Scanning - Anti-CD23 antibody [EPR3617] (ab92495)

        We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
        Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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