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AB135386

Anti-CD23 antibody [SP163]

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(4 Publications)

Rabbit Recombinant Monoclonal CD23 antibody. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] (AB135386)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] (AB135386)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] (AB135386)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] (AB135386)

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD23 with ab135386 antibody at a dilution of 1/50.

Flow Cytometry - Anti-CD23 antibody [SP163] (AB135386)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD23 antibody [SP163] (AB135386)

Flow cytometric analysis of rabbit anti-CD23 (SP163) antibody ab135386 (1/100) in Raji (Human Burkitt's lymphoma cell line) cells (green) compared to negative control of rabbit IgG (blue).

  • Carrier free

    Anti-CD23 antibody [SP163] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP163

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/25", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/25", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.
Biological function summary

CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

Pathways

CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells (PubMed : 2167225). On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed : 7544003).
See full target information FCER2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 56:434-441 PubMed40599290

2025

[ Somatic Mutations Are Associated With Lymph Node Metastasis in Endometrial Cancer].

Applications

Unspecified application

Species

Unspecified reactive species

Qingyu Shen,Chenfan Tian,Xiaoxiao Luo,Fan Yang,Peng Jiang,Yunfeng Zheng

Frontiers in oncology 15:1480461 PubMed39926286

2025

Mature tertiary lymphoid structure associated CD103+ CD8+ Trm cells determined improved anti-tumor immune in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Fang,Shuru Chen,Xiaoyue Chen,Wei Zou,Di Chen,Yukang Huang,Chucheng Wu

BMC cancer 24:758 PubMed38914961

2024

Constructing a prognostic model for colon cancer: insights from immunity-related genes.

Applications

Unspecified application

Species

Unspecified reactive species

Ansu Li,Qi Li,Chaoshan Wang,Xue Bao,Feng Sun,Xiaoping Qian,Wu Sun

Journal for immunotherapy of cancer 11: PubMed37072348

2023

Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miao He,Qihua He,Xiuyu Cai,Jun Liu,Hongshen Deng,Feng Li,Ran Zhong,Yi Lu,Haoxin Peng,Xiangrong Wu,Zisheng Chen,Shen Lao,Caichen Li,Jianfu Li,Jianxing He,Wenhua Liang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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