Rabbit Recombinant Monoclonal CD23 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732), expandable thumbnail](https://content.abcam.com/products/images/cd23-antibody-sp163-bsa-and-azide-free-ab245732--flow-cytometry-img123258.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732), expandable thumbnail](https://content.abcam.com/products/images/cd23-antibody-sp163-bsa-and-azide-free-ab245732--immunocytochemistry-immunofluorescence-img16995.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732), expandable thumbnail](https://content.abcam.com/products/images/cd23-antibody-sp163-bsa-and-azide-free-ab245732--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139422.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] - BSA and Azide free (AB245732)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells (PubMed:2167225). On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed:7544003).
Alternative names
CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor
Recommended products
Rabbit Recombinant Monoclonal CD23 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP163
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab245732 is the carrier-free version of Anti-CD23 antibody [SP163] ab135386.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.
- Biological function summary
CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.
- Pathways
CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.
- Associated diseases and disorders
CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732), expandable thumbnail](https://content.abcam.com/products/images/cd23-antibody-sp163-bsa-and-azide-free-ab245732--flow-cytometry-img123258.jpg "Flow Cytometry - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732)")
Flow Cytometry - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD23 with Anti-CD23 antibody [SP163] ab135386 antibody at a dilution of 1/50.This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (Anti-CD23 antibody [SP163] ab135386)
![Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732), expandable thumbnail](https://content.abcam.com/products/images/cd23-antibody-sp163-bsa-and-azide-free-ab245732--immunocytochemistry-immunofluorescence-img16995.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732)")
Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732)
Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified Anti-CD23 antibody [SP163] ab135386 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD23 antibody [SP163] ab135386).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732), expandable thumbnail](https://content.abcam.com/products/images/cd23-antibody-sp163-bsa-and-azide-free-ab245732--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139422.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] - BSA and Azide free (ab245732)
Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD23 with Anti-CD23 antibody [SP163] ab135386 antibody at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD23 antibody [SP163] ab135386).
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com